Pascual, L.; El Sayed Shehata Nasr, S.; Marcos Martínez, MD.; Martínez-Máñez, R.; Sancenón Galarza, F. (2017). Acetylcholinesterase-capped Mesoporous Silica Nanoparticles Controlled by the Presence of Inhibitors. Chemistry - An Asian Journal. 12(7):775-784. doi:10.1002/asia.201700031
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/99648
Title:
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Acetylcholinesterase-capped Mesoporous Silica Nanoparticles Controlled by the Presence of Inhibitors
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Author:
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Pascual, Lluís
El Sayed Shehata Nasr, Sameh
Marcos Martínez, María Dolores
Martínez-Máñez, Ramón
Sancenón Galarza, Félix
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UPV Unit:
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Universitat Politècnica de València. Departamento de Química - Departament de Química
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Issued date:
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Abstract:
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[EN] Two different acetylcholinesterase (AChE)-capped mesoporous silica nanoparticles (MSNs), S1-AChE and S2-AChE, were prepared and characterized. MSNs were loaded with rhodamine B and the external surface was functionalized ...[+]
[EN] Two different acetylcholinesterase (AChE)-capped mesoporous silica nanoparticles (MSNs), S1-AChE and S2-AChE, were prepared and characterized. MSNs were loaded with rhodamine B and the external surface was functionalized with either pyridostigmine derivative P1 (to yield solid S1) or neostigmine derivative P2 (to obtain S2). The final capped materials were obtained by coordinating grafted P1 or P2 with AChE ' s active sites (to give S1-AChE and S2-AChE, respectively). Both materials were able to release rho-damine B in the presence of diisopropylfluorophosphate (DFP) or neostigmine in a concentration-dependent manner via the competitive displacement of AChE through DFP and neostigmine coordination with the AChE ' s active sites. The responses of S1-AChE and S2-AChE were also tested with other enzyme inhibitors and substrates. These studies suggest that S1-AChE nanoparticles can be used for the selective detection of nerve agent simulant DFP and paraoxon.
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Subjects:
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Acetylcholinesterase
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Enzyme inhibitors
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Enzyme-capped nanoparticles
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Neostigmine
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Nerve agent simulants
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Chemistry - An Asian Journal. (issn:
1861-4728
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DOI:
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10.1002/asia.201700031
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Publisher:
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John Wiley & Sons
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Publisher version:
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https://doi.org/10.1002/asia.201700031
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Project ID:
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GV/PROMETEOII/2014/047
MINECO/MAT2015-64139-C4-1-R
MINECO/AGL2015-70235-C2-2-R
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Thanks:
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Financial support from the Spanish Government and FEDER funds (Project MAT2015‐64139‐C4‐1‐R, AGL2015‐70235‐C2‐2‐R) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. Ll. P. is grateful ...[+]
Financial support from the Spanish Government and FEDER funds (Project MAT2015‐64139‐C4‐1‐R, AGL2015‐70235‐C2‐2‐R) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. Ll. P. is grateful to the Universitat Politécnica de Valencia for his grant.
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Type:
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Artículo
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