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Browsing by Author "Carbonell Olivares, Alberto"

Now showing items 21-31 of 31

Preparation of multiplexed small RNA libraries from plants

Gilbert, Kerrigan B.; Fahlgren, Noah; Kasschau, Kristin D.; Chapman, Elisabeth J.; Carrington, James C.; CARBONELL, ALBERTO (Bio-Protocol, 2014-11-05)

[EN] High-throughput sequencing is a powerful tool for exploring small RNA populations in plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing multiple samples while still obtaining ...
Resistencia a viroides inducida por ribozimas de cabeza de martillo y RNAs interferentes

Carbonell Olivares, Alberto (Universitat Politècnica de València, 2008-05-07)

Los viroides, los agentes infecciosos conocidos más simples, están constituidos por una molécula circular de RNA monocatenario. A pesar de su pequeño tamaño (246-401 nt) y de no codificar proteínas, son capaces de replicarse ...
Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

Garcia-Ruiz, Hernan; CARBONELL, ALBERTO; Hoyer, J. Steen; Fahlgren, Noah; Gilbert, Kerrigan B.; Takeda, Atsushi; Giampetruzzi, Annalisa; García Ruíz, Maira T.; McGinn, Michaela G.; Lowery, Nicholas; Martínez Baladejo, María T.; Carrington, James C. (Public Library of Science, 2015-03)

[EN] In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a ...
Silenciamiento de genes de Nicotiana benthamiana mediante la expresión de precursores de pequeños ARNs artificiales incluidos en un ARN viroidal

Cantero Sánchez, José Luis (Universitat Politècnica de València, 2021-02-16)

[ES] El silenciamiento génico mediado por RNA o interferencia por RNA (RNAi) es un conjunto de mecanismos de regulación de la expresión génica presente en la mayoría de grandes grupos taxonómicos, entre ellos las plantas, ...
Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo

MINOIA, SOFIA; CARBONELL, ALBERTO; Di Serio, Francesco; Gisel, Andreas; Carrinton, James C.; Navarro, Beatriz; FLORES PEDAUYE, RICARDO (American Society for Microbiology, 2014-10)
Symptomatic plant viroid infections in phytopathogenic fungi: a request for a critical reassessment

Serra, Pedro; CARBONELL, ALBERTO; Navarro, Beatriz; Gago Zachert, Selma; Li, Shigang; Di Serio, Francesco; FLORES PEDAUYE, RICARDO (Proceedings of the National Academy of Sciences, 2020-05-12)
The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner

CARBONELL, ALBERTO; Dujovny, Gabriela; Garcia, Juan Antonio; Valli, Adrian (Scientific Societies, 2012-02)

[EN] Plant viruses of the genera Potyvirus and Ipomovirus (Potyviridae family) use unrelated RNA silencing suppressors (RSS) to counteract antiviral RNA silencing responses. HCPro is the RSS of Potyvirus spp., and its ...
The Potyviridae P1a leader protease contributes to host range specificity

Shan, Hongying; Pasin, Fabio; Valli, Adrian; Castillo, Carla; Rajulu, Charukesi; CARBONELL, ALBERTO; Simon-Mateo, Carmen; Garcia, Juan Antonio; Rodamilans, Bernardo (Elsevier, 2015-02)

[EN] The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and ...
Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA

Carbonell, Alberto; Flores Pedauye, Ricardo; Gago Zachert, Selma Persida (Oxford University Press (OUP): Policy C - Option B, 2011)

[EN] Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the ...
Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis

Cuperus, Josh T.; CARBONELL, ALBERTO; Fahlgren, Noah; Garcia-Ruiz, Hernan; Burke, Russell T.; Takeda, Atsushi; Sullivan, Christopher M.; Gilbert, Sunny D.; Montgomery, Taiowa A.; Carrington, James C. (Nature Publishing Group, 2010-08)

[EN] NA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNAs (miRNAs). Here, ...
Virus variants with differences in the P1 protein coexist in a Plum pox virus population and display particular host-dependent pathogenicity features

Maliogka, Varvara I.; Salvador, Beatriz; CARBONELL, ALBERTO; Saenz, Pilar; San Leon, David; Oliveros, Juan Carlos; Delgadillo, Ma Otilia; García, Juan Antonio; Simon-Mateo, Carmen (Blackwell Publishing, 2012-10)

[EN] Subisolates segregated from an M-type Plum pox virus (PPV) isolate, PPV-PS, differ widely in pathogenicity despite their high degree of sequence similarity. A single amino acid substitution, K109E, in the helper ...