ARGONAUTE (AGO) proteins function in small RNA (sRNA)-based RNA silencing pathways to regulate gene expression and control invading nucleic acids. In posttranscriptional RNA silencing pathways, plant AGOs associate with ...
Pallás Benet, Vicente; HERNANDEZ FORT, CARMEN; Marcos, Jose F.; Daròs, José-Antonio; Ambrós, Silvia; Navarro, Beatriz; NAVARRO BOHIGUES, JOSE ANTONIO; La Peña Del Rivero, Marcos De; Gago-Zachert, Selma; Gas, Maria E.; CARBONELL, ALBERTO; López Del Rincón, Carmelo; Martínez-De Alba, Angel E.; Di Serio, Francesco; Moreno, Pedro(Elsevier, 2022-04-15)
[EN] Ricardo Flores (1947-2020) focused his research on the identification, replication, pathogenesis, and evolution of viroids, the minimal non-protein-coding circular RNAs (250-400 nt) able to replicate and incite diseases ...
[EN] MicroRNA (miRNA)-mediated cleavage is involved in numerous essential cellular pathways. miRNAs recognize target RNAs via sequence complementarity. In addition to complementarity, in vitro and in silico studies have ...
[EN] RNA interference (RNAi)-based tools are used in multiple organisms to induce antiviral resistance through the sequence-specific degradation of target RNAs by complementary small RNAs. In plants, highly specific antiviral ...
CARBONELL, ALBERTO; Takeda, Atsushi; Fahlgren, Noah; Johnson, Simon C.; Cuperus, Josh T.; Carrington, James C.(American Society of Plant Biologists, 2014-05)
[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are used for small RNA-based, specific gene silencing or knockdown in plants. Current methods to generate amiRNA or ...
Fahlgren, Noah; Hill, Steven T.; Carrington, James C.; CARBONELL, ALBERTO(Oxford University Press, 2016-01-01)
[EN] The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated
design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syntasiRNAs)
for efficient and specific ...
ARGONAUTEs (AGOs) are the effector proteins in eukaryotic small RNA(sRNA)–
based gene silencing pathways controlling gene expression and transposon activity. In
plants, AGOs regulate key biological processes such as ...
Gilbert, Kerrigan B.; Fahlgren, Noah; Kasschau, Kristin D.; Chapman, Elisabeth J.; Carrington, James C.; CARBONELL, ALBERTO(Bio-Protocol, 2014-11-05)
[EN] High-throughput sequencing is a powerful tool for exploring small RNA populations in
plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing
multiple samples while still obtaining ...
Carbonell Olivares, Alberto(Universitat Politècnica de València, 2008-05-07)
Los viroides, los agentes infecciosos conocidos más simples, están constituidos por una molécula circular de RNA monocatenario. A pesar de su pequeño tamaño (246-401 nt) y de no codificar proteínas, son capaces de replicarse ...
[EN] Viroids are small (250-400 nucleotides), single-stranded, circular RNAs without protein-coding capacity that infect a large number of ornamental and crop plant species, causing high economic losses worldwide. Strategies ...
Di Serio, Francesco; Owens, Robert A.; Navarro, Beatriz; Serra, Pedro; Martínez De Alba, Angel Emilio; Delgado Villar, Sonia Guadalupe; CARBONELL, ALBERTO; Gago-Zachert, Selma(Elsevier, 2023-01-02)
[EN] Viroids are small, single-stranded, non-protein coding and circular RNAs able to infect host plants in the absence of any helper virus. They may elicit symptoms in their hosts, but the underlying molecular pathways ...
Garcia-Ruiz, Hernan; CARBONELL, ALBERTO; Hoyer, J. Steen; Fahlgren, Noah; Gilbert, Kerrigan B.; Takeda, Atsushi; Giampetruzzi, Annalisa; García Ruíz, Maira T.; McGinn, Michaela G.; Lowery, Nicholas; Martínez Baladejo, María T.; Carrington, James C.(Public Library of Science, 2015-03)
[EN] In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a ...
CARBONELL, ALBERTO(Frontiers Media SA, 2019-05-28)
[EN] In plants, RNA silencing regulates key biological processes such as development, response to stress, genome integrity, and antiviral resistance. RNA silencing functions through diverse classes of small RNAs (sRNAs) ...
Cantero Sánchez, José Luis(Universitat Politècnica de València, 2021-02-16)
[ES] El silenciamiento génico mediado por RNA o interferencia por RNA (RNAi) es un conjunto de mecanismos de regulación de la expresión génica presente en la mayoría de grandes grupos taxonómicos, entre ellos las plantas, ...
Serra, Pedro; CARBONELL, ALBERTO; Navarro, Beatriz; Gago Zachert, Selma; Li, Shigang; Di Serio, Francesco; FLORES PEDAUYE, RICARDO(Proceedings of the National Academy of Sciences, 2020-05-12)
Cisneros, Adriana E.; De la Torre-Montaña, Ainhoa; CARBONELL, ALBERTO(Blackwell Publishing, 2022-05)
[EN] Artificial small RNAs (art-sRNAs) are 21-nucleotide small RNAs (sRNAs) computationally designed to silence plant genes or pathogenic RNAs with high efficacy and specificity. They are typically produced in transgenic ...
[EN] Plant viruses of the genera Potyvirus and Ipomovirus (Potyviridae family) use unrelated RNA silencing suppressors (RSS) to counteract antiviral RNA silencing responses. HCPro is the RSS of Potyvirus spp., and its ...
[EN] The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage
serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the
viral polyprotein, and ...
[EN] Plants adapt to changing environments by optimizing the fitness costs associated with key biological functions. A comparison of a laboratory strain with other wild Australian accessions of Nicotiana benthamiana reveals ...