Cordero-Cucart, María Teresa; Aragonés, V.; Daros Arnau, Jose Antonio(Journal of Visualized Experiments, 2018)
[EN] With increasing interest in RNA biology and the use of RNA molecules in sophisticated biotechnological applications, the methods to produce large amounts of recombinant RNAs are limited. Here, we describe a protocol ...
De la Iglesia Jordán, Francisca; Martinez Garcia, Fernando; Hillung, Julia; Cuevas Torrijos, Jose Manuel; Gerrish, Philip J.; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(American Society for Microbiology, 2012)
[EN] A potential drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs is the ease with which viruses may generate escape mutations. Using a variation of the classic Luria-Delbruck ...
Majer, Eszter(Universitat Politècnica de València, 2016-09-01)
[EN] Plant viruses are obligate intracellular parasites which were used to develop recombinant plant virus vectors to express heterologous proteins and to modify endogenous metabolic pathways of natural products in plants. ...
Rodrigo, Guillermo; Prakash, Satya; Shen, Shensi; Majer, Eszter; DAROS ARNAU, JOSE ANTONIO; Jaramillo, Alfonso(Oxford University Press, 2017-09-19)
[EN] Synthetic gene circuits allow the behavior of living cells to be reprogrammed, and non-coding small RNAs (sRNAs) are increasingly being used as programmable regulators of gene expression. However, sRNAs (natural or ...
[EN] As genomic architectures become more complex, they begin to accumulate degenerate and redundant elements. However, analyses of the molecular mechanisms underlying these genetic architecture features remain scarce, ...
[EN] RNA interference (RNAi)-based tools are used in multiple organisms to induce antiviral resistance through the sequence-specific degradation of target RNAs by complementary small RNAs. In plants, highly specific antiviral ...
Cordero-Cucart, María Teresa; Ortolá-Navarro, Beltrán; Daros Arnau, Jose Antonio(Frontiers Media SA, 2018)
[EN] Eggplant latent viroid (ELVd) is a relatively small non-coding circular RNA that induces asymptomatic infections in eggplants (Solanum melongena L.). Like other viroid species that belong to the family Avsunviroidae, ...
Zwart, Mark Peter; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(Public Library of Science, 2011)
[EN] Effective population size (N-e) determines the strength of genetic drift and the frequency of co-infection by multiple genotypes, making it a key factor in viral evolution. Experimental estimates of N-e for different ...
Martí Botella, Mari Carmen(Universitat Politècnica de València, 2022-10-06)
[ES] La biotecnología de plantas actual, y la llamada agricultura molecular aspiran a convertir las plantas en "biofábricas" sostenibles para producir compuestos de valor como proteínas, metabolitos o nanopartículas de ...
Chucuri Malan, Juan José(Universitat Politècnica de València, 2023-12-27)
[ES] La astaxantina es un carotenoide de gran importancia utilizado en la industria alimentaria. Suscita además un interés debido a sus potenciales aplicaciones como antioxidante en campos como la medicina y la farmacología. ...
Monzó Donat, María Inmaculada(Universitat Politècnica de València, 2016-07-08)
[EN] Here, we have analyzed the possibility of obtaining the three human vault RNAs using a recombinant RNA production system in Escherichia coli, which is based on the use of eggplant latent viroid as a molecular scaffold.The ...
Lozano Sánchez, Enrique(Universitat Politècnica de València, 2022-03-30)
[ES] El uso biotecnológico de nanopartículas virales (VNPs) suscita un gran interés debido a sus potenciales aplicaciones biotecnológicas en campos como la medicina o la nanotecnología. Las VNPs permiten la presentación ...
Martí, Mari Carmen; Merwaiss, Fernando; Butkovic, Anamarija; Daròs, José-Antonio(Frontiers Media S.A., 2022-03-31)
[EN] Viral nanoparticles (VNPs) have recently attracted attention for their use as building blocks for novel materials to support a range of functions of potential interest in nanotechnology and medicine. Viral capsids are ...
Nicolau Sanus, María(Universitat Politècnica de València, 2023-11-30)
[ES] Las plantas están emergiendo como una alternativa atractiva a los sistemas convencionales de producción heteróloga, como bacterias, cultivos celulares de mamíferos, levaduras u hongos, para la síntesis de productos ...
Pasin, Fabio; Daròs, José-Antonio; Tzanetakis, Ioannis E.(Oxford University Press, 2022-07-01)
[EN] Potyviridae represents one of the most notable evolutionary radiations among RNA viruses; its species richness is accompanied by large genomic layout diversity and expansion of the encoded protein modules.
Potyviridae, ...
[EN] Composed of a few hundreds of nucleotides, viroids are infectious, circular, non-protein coding RNAs able to usurp plant cellular enzymes and molecular machineries to replicate and move in their hosts. Several secondary ...
[EN] Sustainable use of nanotechnology in crop protection requires an understanding of the plant's life cycle, potential toxicological impacts of nanomaterials and their mechanism of action against the target pathogens. ...
Majer, Eszter; Salvador, Zaira; Zwart, Mark Peter; Willemsen, Anouk; Elena Fito, Santiago Fco; Daros Arnau, Jose Antonio(American Society for Microbiology, 2014-04)
[EN] Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, ...
[EN] Plants can be engineered to sustainably produce compounds of nutritional, industrial or pharmaceutical relevance. This is, however, a challenging task as extensive regulation of biosynthetic pathways often hampers ...
Eiras, Marcelo; Nohales Zafra, Maria Angeles; Kitajima, Elliot J.; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio(Springer Verlag (Germany), 2011-03)
[EN] Potato spindle tuber viroid (PSTVd) contains an element of tertiary structure -loop E- also present in eukaryotic 5S rRNA. The ribosomal protein L5 and transcription factor IIIA (TFIIIA) from Arabidopsis thaliana bind ...