Cantero Sánchez, José Luis(Universitat Politècnica de València, 2021-02-16)
[ES] El silenciamiento génico mediado por RNA o interferencia por RNA (RNAi) es un conjunto de mecanismos de regulación de la expresión génica presente en la mayoría de grandes grupos taxonómicos, entre ellos las plantas, ...
[EN] Viral vectors provide a quick and effective way to express exogenous sequences in eukaryotic cells and to engineer eukaryotic genomes through the delivery of CRISPR/Cas components. Here, we presentJoinTRV, an improved ...
Majer, Eszter; Daros Arnau, Jose Antonio; Zwart, Mark Peter(MDPI, 2013-09)
[EN] Antirrhinum majus Rosea1 (Ros1) is an MYB-related transcription factor that
induces anthocyanin biosynthesis in plant tissues, and has been shown to be suitable for
visual tracking of virus infection in plants. ...
[EN] CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This ...
Gobatto, Danielle; de Oliveira, Lucas Araujo; de Siqueira Franco, Daniel Andrade; Velasquez, Nubia; Daròs, José-Antonio; EIRAS, MARCELO(MDPI AG, 2019-04-17)
[EN] The stunting disease, incited by chrysanthemum stunt viroid (CSVd), has become a serious problem in chrysanthemum production areas worldwide. Here we identified 46 weed species from chrysanthemum fields in two producing ...
Llorente, Briardo; Torres-Montilla, Salvador; Morelli, Luca; Florez-Sarasa, Igor; Matus, José Tomás; Ezquerro, Miguel; D'Andrea, Lucio; Houhou, Fakhreddine; Majer, Eszter; Picó Sirvent, María Belén; Cebolla Cornejo, Jaime; Troncoso, Adrian; Fernie, Alisdair R.; Daròs, José-Antonio; Rodriguez-Concepcion, Manuel(Proceedings of the National Academy of Sciences, 2020-09-01)
[EN] Plastids, the defining organelles of plant cells, undergo physiological and morphological changes to fulfill distinct biological functions. In particular, the differentiation of chloroplasts into chromoplasts results ...
Lafforgue, Guillaume; Martínez García, Fernando; Sardanyes Cayuela, Jose; De la Iglesia Jordán, Francisca; Niu, Qi-Wen; Lin, Shih-Shun; Sole, Ricard V.; Chua, Nam-Hai; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(American Society for Microbiology, 2011)
A biotechnological application of artificial microRNAs (amiRs) is the generation of plants that are resistant to virus infection. This resistance has proven to be highly effective and sequence specific. However, before ...
Contreras-Paredes, Carlos A.; Silva-Rosales, Laura; Daros Arnau, Jose Antonio; Alejandri-Ramirez, Naholi D.; Dinkova, Tzvetanka D.(American Phytopathological Society, 2013-04)
[EN] Translation initiation factor eIF4E exerts an important role during infection of viral species in the family Potyviridae. Particularly, a eIF(iso)4E family member is required for Arabidopsis thaliana susceptibility ...
Marques, Jorge; Duran Vila, Nuria; Daros Arnau, Jose Antonio(Elsevier, 2011-04)
[EN] Brittle leaf disease or maladie des feuilles cassantes (MFC) is a disorder affecting date palms (Phoenix dactylifera L) which after a long declining process eventually leads to the death of the plant. No causal agent ...
Martínez, Fernando; Daros Arnau, Jose Antonio(American Society for Microbiology, 2014-09)
[EN] The genus Potyvirus comprises a large group of positive-strand RNA plant viruses whose genome encodes a large polyprotein
processed by three viral proteinases. P1 protein, the most amino-terminal product of the ...
[EN] Artificial microRNAs (amiRNAs) are highly specific, 21-nucleotide (nt) small RNAs designed to silence target transcripts. In plants, their application as biotechnological tools for functional genomics or crop improvement ...
[EN]
Enrichment of foodstuffs with health-promoting metabolites such as carotenoids is a powerful tool to fight against unhealthy eating habits. Dietary carotenoids are vitamin A precursors and reduce risk of several ...
Martinez Garcia, Fernando; Lafforgue, Guillaume; Morelli, M.; González-Candelas, F.; CHUA, N.H.; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(Oxford University Press (OUP), 2012-11)
[EN] Plant artificial micro-RNAs (amiRs) have been engineered to target viral genomes and induce their degradation. However, the exceptional evolutionary plasticity of RNA viruses threatens the durability of the resistance ...
[EN] Plants defend themselves from invading viruses using RNA silencing. However, plant viruses try to spoil this defensive mechanism by expressing one or more proteins that act as RNA silencing suppressors. One such protein ...
[EN] Viroids, due to their small size and lack of protein-coding capacity, must rely essentially on their hosts for replication. Intriguingly, viroids have evolved the ability to replicate in two cellular organella, the ...
Nohales Zafra, Maria Angeles; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio(National Academy of Sciences, 2012-08-21)
[EN] Viroids are a unique class of noncoding RNAs: composed of only a circular, single-stranded molecule of 246-401 nt, they manage to replicate, move, circumvent host defenses, and frequently induce disease in higher ...
Rodrigo Tarrega, Guillermo; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(Elsevier, 2017)
[EN] Viral infections are extremely complex processes that could only be well understood by precisely characterizing the interaction networks between the virus and the host components. In recent years, much effort has gone ...
Bedoya, Leonor C.; Martinez Garcia, Fernando; Orzáez Calatayud, Diego Vicente; Daros Arnau, Jose Antonio(American Society of Plant Biologists, 2012-03)
Insertion of reporter genes into plant virus genomes is a common experimental strategy to research many aspects of the viral infection dynamics. Their numerous advantages make fluorescent proteins the markers of choice in ...