Lafforgue, G.; Martínez García, F.; Sardanyes Cayuela, J.; De La Iglesia Jordán, F.; Niu, Q.; Lin, S.; Sole, RV.... (2011). Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance. Journal of Virology. 85(19):9686-9695. doi:10.1128/JVI.05326-11
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/34546
Title:
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Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance
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Author:
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Lafforgue, Guillaume
Martínez García, Fernando
Sardanyes Cayuela, Jose
De la Iglesia Jordán, Francisca
Niu, Qi-Wen
Lin, Shih-Shun
Sole, Ricard V.
Chua, Nam-Hai
Daros Arnau, Jose Antonio
Elena Fito, Santiago Fco
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UPV Unit:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
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Issued date:
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Abstract:
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A biotechnological application of artificial microRNAs (amiRs) is the generation of plants that are resistant to virus infection. This resistance has proven to be highly effective and sequence specific. However, before ...[+]
A biotechnological application of artificial microRNAs (amiRs) is the generation of plants that are resistant to virus infection. This resistance has proven to be highly effective and sequence specific. However, before these transgenic plants can be deployed in the field, it is important to evaluate the likelihood of the emergence of resistance-breaking mutants. Two issues are of particular interest: (i) whether such mutants can arise in nontransgenic plants that may act as reservoirs and (ii) whether a suboptimal expression level of the transgene, resulting in subinhibitory concentrations of the amiR, would favor the emergence of escape mutants. To address the first issue, we experimentally evolved independent lineages of Turnip mosaic virus (TuMV) (family Potyviridae) in fully susceptible wild-type Arabidopsis thaliana plants and then simulated the spillover of the evolving virus to fully resistant A. thaliana transgenic plants. To address the second issue, the evolution phase took place with transgenic plants that expressed the amiR at subinhibitory concentrations. Our results show that TuMV populations replicating in susceptible hosts accumulated resistance-breaking alleles that resulted in the overcoming of the resistance of fully resistant plants. The rate at which resistance was broken was 7 times higher for TuMV populations that experienced subinhibitory concentrations of the antiviral amiR. A molecular characterization of escape alleles showed that they all contained at least one nucleotide substitution in the target sequence, generally a transition of the G-to-A and C-to-U types, with many instances of convergent molecular evolution. To better understand the viral population dynamics taking place within each host, as well as to evaluate relevant population genetic parameters, we performed in silico simulations of the experiments. Together, our results contribute to the rational management of amiR-based antiviral resistance in plants.
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Subjects:
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Cucumber-mosaic-virus
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Antiretroviral resistance
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Viral-RNA
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Type-1
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Inhibition
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Replication
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Arabidopsis
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Mutations
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Efficient
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Transcription
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Journal of Virology. (issn:
0022-538X
)
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DOI:
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10.1128/JVI.05326-11
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Publisher:
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American Society for Microbiology
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Publisher version:
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http://dx.doi.org/10.1128/JVI.05326-11
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Project ID:
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Human Frontiers Science Program Organization RGP12/2008
Generalitat Valenciana PROMETEO/2010/019
CSIC 2010TW0015
Santa Fe Institute
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Thanks:
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This work was supported by Human Frontiers Science Program Organization grant RGP12/2008, Generalitat Valenciana grant PROMETEO/2010/019, and CSIC grant 2010TW0015. We also acknowledge support from The Santa Fe Institute.[+]
This work was supported by Human Frontiers Science Program Organization grant RGP12/2008, Generalitat Valenciana grant PROMETEO/2010/019, and CSIC grant 2010TW0015. We also acknowledge support from The Santa Fe Institute.
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Type:
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Artículo
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