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Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance.

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Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance.

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dc.contributor.author Lafforgue, Guillaume es_ES
dc.contributor.author Martínez García, Fernando es_ES
dc.contributor.author Sardanyes Cayuela, Jose es_ES
dc.contributor.author De la Iglesia Jordán, Francisca es_ES
dc.contributor.author Niu, Qi-Wen es_ES
dc.contributor.author Lin, Shih-Shun es_ES
dc.contributor.author Sole, Ricard V. es_ES
dc.contributor.author Chua, Nam-Hai es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2013-12-16T13:44:15Z
dc.date.available 2013-12-16T13:44:15Z
dc.date.issued 2011
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/34546
dc.description.abstract A biotechnological application of artificial microRNAs (amiRs) is the generation of plants that are resistant to virus infection. This resistance has proven to be highly effective and sequence specific. However, before these transgenic plants can be deployed in the field, it is important to evaluate the likelihood of the emergence of resistance-breaking mutants. Two issues are of particular interest: (i) whether such mutants can arise in nontransgenic plants that may act as reservoirs and (ii) whether a suboptimal expression level of the transgene, resulting in subinhibitory concentrations of the amiR, would favor the emergence of escape mutants. To address the first issue, we experimentally evolved independent lineages of Turnip mosaic virus (TuMV) (family Potyviridae) in fully susceptible wild-type Arabidopsis thaliana plants and then simulated the spillover of the evolving virus to fully resistant A. thaliana transgenic plants. To address the second issue, the evolution phase took place with transgenic plants that expressed the amiR at subinhibitory concentrations. Our results show that TuMV populations replicating in susceptible hosts accumulated resistance-breaking alleles that resulted in the overcoming of the resistance of fully resistant plants. The rate at which resistance was broken was 7 times higher for TuMV populations that experienced subinhibitory concentrations of the antiviral amiR. A molecular characterization of escape alleles showed that they all contained at least one nucleotide substitution in the target sequence, generally a transition of the G-to-A and C-to-U types, with many instances of convergent molecular evolution. To better understand the viral population dynamics taking place within each host, as well as to evaluate relevant population genetic parameters, we performed in silico simulations of the experiments. Together, our results contribute to the rational management of amiR-based antiviral resistance in plants. es_ES
dc.description.sponsorship This work was supported by Human Frontiers Science Program Organization grant RGP12/2008, Generalitat Valenciana grant PROMETEO/2010/019, and CSIC grant 2010TW0015. We also acknowledge support from The Santa Fe Institute. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation Human Frontiers Science Program Organization RGP12/2008 es_ES
dc.relation Generalitat Valenciana PROMETEO/2010/019 es_ES
dc.relation CSIC 2010TW0015 es_ES
dc.relation Santa Fe Institute es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cucumber-mosaic-virus es_ES
dc.subject Antiretroviral resistance es_ES
dc.subject Viral-RNA es_ES
dc.subject Type-1 es_ES
dc.subject Inhibition es_ES
dc.subject Replication es_ES
dc.subject Arabidopsis es_ES
dc.subject Mutations es_ES
dc.subject Efficient es_ES
dc.subject Transcription es_ES
dc.title Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.05326-11
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Lafforgue, G.; Martínez García, F.; Sardanyes Cayuela, J.; De La Iglesia Jordán, F.; Niu, Q.; Lin, S.; Sole, RV.... (2011). Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance. Journal of Virology. 85(19):9686-9695. doi:10.1128/JVI.05326-11 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/JVI.05326-11 es_ES
dc.description.upvformatpinicio 9686 es_ES
dc.description.upvformatpfin 9695 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 85 es_ES
dc.description.issue 19 es_ES
dc.relation.senia 216049
dc.identifier.pmid 21775453 en_EN
dc.identifier.pmcid PMC3196453 en_EN


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