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Browsing by Subject "Androgenesis"

Now showing items 1-20 of 29

A Deep Learning-Based System (Microscan) for the Identification of Pollen Development Stages and Its Application to Obtaining Doubled Haploid Lines in Eggplant

García-Fortea, Edgar; García-Pérez, Ana; Gimeno -Páez, Esther; Sánchez-Gimeno, Alfredo; Vilanova Navarro, Santiago; Prohens Tomás, Jaime; Pastor-Calle, David (MDPI AG, 2020-09)

[EN] The development of double haploids (DHs) is a straightforward path for obtaining pure lines but has multiple bottlenecks. Among them is the determination of the optimal stage of pollen induction for androgenesis. In ...
Assessment of different anther culture approaches to produce doubled haploids in cucumber (Cucumis sativus L.)

Asadi, Abouzar; Zebarjadi, Alireza; Abdollahi, Mohammad Reza; Seguí-Simarro, Jose M. (Springer-Verlag, 2018)

[EN] Cucumber is one of the most important vegetable crops worldwide, which makes it a good candidate to produce doubled haploid (DH) lines to accelerate plant breeding. Traditionally, these approaches involved induction ...
Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

Rivas Sendra, Alba (Universitat Politècnica de València, 2017-07-20)

Androgenesis induction is an experimental procedure by which microspores are diverted from their original gametophytic pathway towards embryogenesis by applying specific stresses in vitro. It allows for the production of ...
Caracterización de la transición de embrión globular a corazón en embriones androgénicos de berenjena

García Fortea, Edgar (Universitat Politècnica de València, 2015-09-04)

[ES] La puesta a punto de métodos eficientes de obtención de individuos doble haploides (DHs) es uno de los objetivos principales de muchas empresas empresas de mejora genética, pues permiten obtener líneas puras de forma ...
Cell Wall Composition and Structure Define the Developmental Fate of Embryogenic Microspores in Brassica napus

Camacho-Fernández, Carolina; Seguí-Simarro, Jose M.; Mir Moreno, Ricardo; Boutilier, Kim; Corral-Martínez, Patricia (Frontiers Media SA, 2021-10-06)

[EN] Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic ...
Cultivo de anteras y microsporas aisladas con extractos purificados de endospermo líquido de Cocos nucifera

Tur Giménez, Laura (Universitat Politècnica de València, 2020-10-05)

[ES] La embriogénesis de microsporas consiste en desviar in vitro la ruta normal de desarrollo gametofítico de las microsporas para formar un embrión a partir del cual se obtendrán plantas haploides o dobles haploides tras ...
Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response

Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Springer-Verlag, 2017)

[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Doubled haploids in eggplant

Mir Moreno, Ricardo; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (MDPI AG, 2021-07)

[EN] This review compiles the most relevant advances made in the production of doubled haploid plants in eggplant, the application of doubled haploid lines in breeding programs, and the future perspectives for the development ...
Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena

Rivas-Sendra, Alba; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Frontiers Media SA, 2017)

[EN] Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels ...
Efecto del ácido abscísico en la respuesta androgénica de microsporas de Brassica napus

Remírez de Ganuza Monfort, María (Universitat Politècnica de València, 2020-09-02)

[ES] La inducción de androgénesis es un proceso por el cual las microsporas o células precursoras del polen son inducidas a formar embriones bajo determinadas condiciones de cultivo in vitro, obteniéndose así plantas ...
Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes

Rivas-Sendra, Alba; Corral Martínez, Patricia; Camacho-Fernández, Carolina; Porcel, R; Seguí-Simarro, Jose M. (Springer-Verlag, 2020-10-06)

[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis ...
Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.)

Corral Martínez, Patricia; Seguí-Simarro, José M. (Springer Verlag (Germany), 2012)

Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore ...
Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer

Rivas-Sendra, Alba; Corral Martínez, Patricia; Porcel, R.; Camacho-Fernández, Carolina; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Oxford University Press, 2019-02-01)

[EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied ...
Enhancing secondary embryogenesis in Brassica napus by selecting hypocotyl-derived embryos and using plant-derived smoke extract in culture medium

Abdollahi, M.R.; Ghazanfari, P.; Corral Martínez, Patricia; Moieni, A.; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2012-08)

Induction of secondary embryogenesis on transformed androgenic microspore-derived embryos (MDEs) is a convenient approach to avoid chimerism and hemizygosis for the introduced transgene. In this work, we improved two aspects ...
Evaluation of androgenic competence through anther culture in common eggplant and related species

Salas Aragon, Pablo; Prohens Tomás, Jaime; Seguí-Simarro, José M. (Springer Verlag (Germany), 2011)

Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 ...
Formation and excretion of autophagic plastids (plastolysomes) in Brassica napus embryogenic microspores

Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M. (Frontiers Media, 2015-02)

[EN] The change in developmental fate of microspores reprogrammed toward embryogenesis is a complex but fascinating experimental system where microspores undergo dramatic changes derived from the developmental switch. ...
Genetic, quantitative and microscopic evidence for fusion of haploid nuclei and growth of somatic calli in cultured ms1035 tomato anthers

Corral Martínez, Patricia; Nuez Viñals, Fernando; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2011-03)

In plant breeding, androgenic doubled haploids represent powerful tools to save time and resources for pure line generation. While in many species efficient protocols are known, in tomato (Solanum lycopersicum), the knowledge ...
Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis

Rivas Sendra, Alba; Corral Martínez, Patricia; Camacho Fernández, Carolina; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2015-09)

[EN] Doubled haploid (DH) technology allows for the production of pure lines, useful for plant breeding, through a one-generation procedure that reduces considerably the time and resources needed to produce them. ...
Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose

Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M. (Frontiers Media, 2015-11-25)

The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this ...
Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures

Salas Aragon, Pablo; Rivas Sendra, Alba; Prohens Tomás, Jaime; Seguí-Simarro, José M. (Springer Verlag (Germany), 2012)

In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly ...