Asadi, Abouzar; Zebarjadi, Alireza; Abdollahi, Mohammad Reza; Seguí-Simarro, Jose M.(Springer-Verlag, 2018)
[EN] Cucumber is one of the most important vegetable crops worldwide, which makes it a good candidate to produce doubled haploid (DH) lines to accelerate plant breeding. Traditionally, these approaches involved induction ...
García Fortea, Edgar(Universitat Politècnica de València, 2015-09-04)
[ES] La puesta a punto de métodos eficientes de obtención de individuos doble haploides (DHs) es uno de los objetivos principales de muchas empresas empresas de mejora genética, pues permiten obtener líneas puras de forma ...
Tur Giménez, Laura(Universitat Politècnica de València, 2020-10-05)
[ES] La embriogénesis de microsporas consiste en desviar in vitro la ruta normal de desarrollo gametofítico de las microsporas para formar un embrión a partir del cual se obtendrán plantas haploides o dobles haploides tras ...
Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Rivas-Sendra, Alba; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Frontiers Media SA, 2017)
[EN] Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels ...
Remírez de Ganuza Monfort, María(Universitat Politècnica de València, 2020-09-02)
[ES] La inducción de androgénesis es un proceso por el cual las microsporas o células precursoras del polen son inducidas a formar embriones bajo determinadas condiciones de cultivo in vitro, obteniéndose así plantas ...
Corral Martínez, Patricia; Seguí-Simarro, José M.(Springer Verlag (Germany), 2012)
Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore ...
Rivas-Sendra, Alba; Corral Martínez, Patricia; Porcel, R.; Camacho-Fernández, Carolina; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Oxford University Press, 2019-02-01)
[EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied ...
Induction of secondary embryogenesis on transformed androgenic microspore-derived embryos (MDEs) is a convenient approach to avoid chimerism and hemizygosis for the introduced transgene. In this work, we improved two aspects ...
Salas Aragon, Pablo; Prohens Tomás, Jaime; Seguí-Simarro, José M.(Springer Verlag (Germany), 2011)
Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 ...
Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M.(Frontiers Media, 2015-02)
[EN] The change in developmental fate of microspores reprogrammed toward embryogenesis
is a complex but fascinating experimental system where microspores undergo dramatic
changes derived from the developmental switch. ...
In plant breeding, androgenic doubled haploids represent powerful tools to save time and resources for pure line generation. While in many species efficient protocols are known, in tomato (Solanum lycopersicum), the knowledge ...
[EN] Doubled haploid (DH) technology allows for
the production of pure lines, useful for plant breeding,
through a one-generation procedure that reduces considerably
the time and resources needed to produce them.
...
Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M.(Frontiers Media, 2015-11-25)
The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this ...
Salas Aragon, Pablo; Rivas Sendra, Alba; Prohens Tomás, Jaime; Seguí-Simarro, José M.(Springer Verlag (Germany), 2012)
In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly ...
Corral Martínez, Patricia; Parra Vega, Verónica; Seguí-Simarro, Jose M.(Oxford University Press (OUP): Policy B - Oxford Open Option A, 2013)
[EN] Induction of embryogenesis from isolated microspore cultures is a complex experimental system where microspores undergo dramatic changes in developmental fate. After ~40 years of application of electron microscopy to ...
Corral Martínez, Patricia(Universitat Politècnica de València, 2011-07-28)
La producción de doble haploides a través del cultivo de microsporas aisladas es una
alternativa para obtener líneas puras mucho más eficaz que las técnicas de mejora genética
clásica. Esta técnica presenta las ventajas ...
Hoveida, Z.S.; Abdollahi, Mohammad Reza; Mirzaie-Asl, Asghar; Moosavi, Sayyed Saeed; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] Anther culture can be used as a powerful tool to produce doubled haploid (DH) lines in medicinal plants, thus accelerating breeding programs. In the particular case of borage (Borago officinalis L.), a method to produce ...
Corral Martínez, Patricia; Seguí-Simarro, Jose M.(Springer Verlag (Germany), 2014-02)
Microspore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce ...
Tomas Buch Belenguer, Irina(Universitat Politècnica de València, 2019-10-01)
[ES] La obtención de híbridos es la base del actual sistema de producción de semillas para la agricultura convencional. Para conseguir híbridos, anteriormente se necesita generar líneas puras, lo que es muy costoso en ...