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Browsing by Subject "Androgenesis"

Now showing items 1-19 of 19

Assessment of different anther culture approaches to produce doubled haploids in cucumber (Cucumis sativus L.)

Asadi, Abouzar; Zebarjadi, Alireza; Abdollahi, Mohammad Reza; Seguí-Simarro, Jose M. (Springer-Verlag, 2018)

[EN] Cucumber is one of the most important vegetable crops worldwide, which makes it a good candidate to produce doubled haploid (DH) lines to accelerate plant breeding. Traditionally, these approaches involved induction ...
Caracterización de la transición de embrión globular a corazón en embriones androgénicos de berenjena

García Fortea, Edgar (2015-09-04)

[ES] La puesta a punto de métodos eficientes de obtención de individuos doble haploides (DHs) es uno de los objetivos principales de muchas empresas empresas de mejora genética, pues permiten obtener líneas puras de forma ...
Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response

Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Springer-Verlag, 2017)

[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena.

Rivas-Sendra, Alba; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Frontiers Media SA, 2017)

[EN] Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels ...
Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.)

Corral Martínez, Patricia; Seguí-Simarro, José M. (Springer Verlag (Germany), 2012)

Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore ...
Enhancing secondary embryogenesis in Brassica napus by selecting hypocotyl-derived embryos and using plant-derived smoke extract in culture medium

Abdollahi, M.R.; Ghazanfari, P.; Corral Martínez, Patricia; Moieni, A.; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2012-08)

Induction of secondary embryogenesis on transformed androgenic microspore-derived embryos (MDEs) is a convenient approach to avoid chimerism and hemizygosis for the introduced transgene. In this work, we improved two aspects ...
Evaluation of androgenic competence through anther culture in common eggplant and related species

Salas Aragon, Pablo; Prohens Tomás, Jaime; Seguí-Simarro, José M. (Springer Verlag (Germany), 2011)

Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 ...
Formation and excretion of autophagic plastids (plastolysomes) in Brassica napus embryogenic microspores

Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M. (Frontiers Media, 2015-02)

[EN] The change in developmental fate of microspores reprogrammed toward embryogenesis is a complex but fascinating experimental system where microspores undergo dramatic changes derived from the developmental switch. ...
Genetic, quantitative and microscopic evidence for fusion of haploid nuclei and growth of somatic calli in cultured ms1035 tomato anthers

Corral Martínez, Patricia; Nuez Viñals, Fernando; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2011-03)

In plant breeding, androgenic doubled haploids represent powerful tools to save time and resources for pure line generation. While in many species efficient protocols are known, in tomato (Solanum lycopersicum), the knowledge ...
Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis

Rivas Sendra, Alba; Corral Martínez, Patricia; Camacho Fernández, Carolina; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2015-09)

[EN] Doubled haploid (DH) technology allows for the production of pure lines, useful for plant breeding, through a one-generation procedure that reduces considerably the time and resources needed to produce them. ...
Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose.

Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M. (Frontiers Media, 2015-11-25)

The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this ...
Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures

Salas Aragon, Pablo; Rivas Sendra, Alba; Prohens Tomás, Jaime; Seguí-Simarro, José M. (Springer Verlag (Germany), 2012)

In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly ...
Novel features of Brassica napus embryogenic microspores revealed by high pressure freezing and freeze substitution: evidence for massive autophagy and excretion-based cytoplasmic cleaning

Corral Martínez, Patricia; Parra Vega, Verónica; Seguí-Simarro, Jose M. (Oxford University Press (OUP): Policy B - Oxford Open Option A, 2013)

[EN] Induction of embryogenesis from isolated microspore cultures is a complex experimental system where microspores undergo dramatic changes in developmental fate. After ~40 years of application of electron microscopy to ...
Obtención de doble haploides en berenjena (Solanum melongena) a partir del cultivo in vitro de microsporas aisladas

Corral Martínez, Patricia (2011-07-28)

La producción de doble haploides a través del cultivo de microsporas aisladas es una alternativa para obtener líneas puras mucho más eficaz que las técnicas de mejora genética clásica. Esta técnica presenta las ventajas ...
Production of doubled haploid plants from anther cultures of borage (Borago officinalis L.) by the application of chemical and physical stress

Hoveida, Z.S.; Abdollahi, Mohammad Reza; Mirzaie-Asl, Asghar; Moosavi, Sayyed Saeed; Seguí-Simarro, Jose M. (Springer-Verlag, 2017)

[EN] Anther culture can be used as a powerful tool to produce doubled haploid (DH) lines in medicinal plants, thus accelerating breeding programs. In the particular case of borage (Borago officinalis L.), a method to produce ...
Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins

Corral Martínez, Patricia; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2014-02)

Microspore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce ...
Relationship between osmolality and in vitro viability in pepper (Capsicum annuum) microspores.

Tomas Buch Belenguer, Irina (2019-10-01)

[ES] La obtención de híbridos es la base del actual sistema de producción de semillas para la agricultura convencional. Para conseguir híbridos, anteriormente se necesita generar líneas puras, lo que es muy costoso en ...
Reprogramación hacia embriogénesis de microsporas en cultivares dulces de pimiento (Capsicum annuum L.) mediante cultivo in vitro de microsporas aisladas

Parra Vega, Verónica (2011-07-28)

En este trabajo se ha desarrollado un protocolo inicial para llevar a cabo la inducción de la androgénesis a partir del cultivo de microsporas aisladas de genotipos dulces de pimiento (Capsicum annuum L.). El objetivo ...
Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.)

Parra Vega, Verónica; Renau Morata, Begoña; Sifres Cuerda, Alicia Gemma; Seguí Simarro, José María (Springer Verlag (Germany), 2013-03)

Production of doubled haploids (DHs) is a convenient tool to obtain pure lines for breeding purposes. Until now, the easiest and most useful approach to obtain pepper DHs is via anther culture. However, this method has an ...