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Immunoprecipitation and High–Throughput Sequencing of ARGONAUTE–Bound Target RNAs from Plants

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Immunoprecipitation and High–Throughput Sequencing of ARGONAUTE–Bound Target RNAs from Plants

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Carbonell Olivares, A. (2017). Immunoprecipitation and High–Throughput Sequencing of ARGONAUTE–Bound Target RNAs from Plants. En Plant Argonaute Proteins: Methods and Protocols. Springer Link. 93-112. https://doi.org/10.1007/978-1-4939-7165-7_6

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Metadatos del ítem

Título: Immunoprecipitation and High–Throughput Sequencing of ARGONAUTE–Bound Target RNAs from Plants
Autor: Carbonell Olivares, Alberto
Editor: Carbonell Olivares, Alberto
Entidad UPV: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Fecha difusión:
Resumen:
ARGONAUTE (AGO) proteins function in small RNA (sRNA)-based RNA silencing pathways to regulate gene expression and control invading nucleic acids. In posttranscriptional RNA silencing pathways, plant AGOs associate with ...[+]
Palabras clave: ARGONAUTE , RNA immunoprecipitation , High-throughput sequencing n , RIP-Seq , Small RNA , RNA-Seq , Library preparatio
Derechos de uso: Reserva de todos los derechos
ISBN: 978-1-4939-7165-7 978-1-4939-7164-0
Fuente:
Plant Argonaute Proteins: Methods and Protocols. (issn: 1064-3745 ) (eissn: 1940-6029 )
DOI: 10.1007/978-1-4939-7165-7_6
Editorial:
Springer Link
Versión del editor: http://dx.doi.org/10.1007/978-1-4939-7165-7_6
Código del Proyecto:
info:eu-repo/grantAgreement/EC/H2020/655841/EU/Genome-wide analysis of RNA and protein interacting profiles during a plant virus infection/
Agradecimientos:
This work was supported by an Individual Fellowship from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 655841 to A.C.
Tipo: Capítulo de libro

References

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Vaucheret H (2008) Plant ARGONAUTES. Trends Plant Sci 13(7):350–358. doi: 10.1016/j.tplants.2008.04.007 [+]
Carbonell A, Carrington JC (2015) Antiviral roles of plant ARGONAUTES. Curr Opin Plant Biol 27:111–117. doi: 10.1016/j.pbi.2015.06.013

Fang X, Qi Y (2016) RNAi in plants: an Argonaute-centered view. Plant Cell 28(2):272–285. doi: 10.1105/tpc.15.00920

Vaucheret H (2008) Plant ARGONAUTES. Trends Plant Sci 13(7):350–358. doi: 10.1016/j.tplants.2008.04.007

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Cuperus JT, Carbonell A, Fahlgren N, Garcia-Ruiz H, Burke RT, Takeda A, Sullivan CM, Gilbert SD, Montgomery TA, Carrington JC (2010) Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. Nat Struct Mol Biol 17(8):997–1003. doi: 10.1038/nsmb.1866

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Carbonell A, Fahlgren N, Garcia-Ruiz H, Gilbert KB, Montgomery TA, Nguyen T, Cuperus JT, Carrington JC (2012) Functional analysis of three Arabidopsis ARGONAUTES using slicer-defective mutants. Plant Cell 24(9):3613–3629. doi: 10.1105/tpc.112.099945

Gilbert KB, Fahlgren N, Kasschau KD, Chapman EJ, Carrington JC, Carbonell A (2014) Preparation of multiplexed small RNA libraries from plants. Bio Protoc 4(21):e1275

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