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Identification and profiling of microRNA between back and belly Skin in Rex rabbits (Oryctolagus cuniculus)

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Identification and profiling of microRNA between back and belly Skin in Rex rabbits (Oryctolagus cuniculus)

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dc.contributor.author Zhao, Bohao es_ES
dc.contributor.author Chen, Yang es_ES
dc.contributor.author Mu, Lin es_ES
dc.contributor.author Hu, Shuaishuai es_ES
dc.contributor.author Wu, Xinsheng es_ES
dc.date.accessioned 2018-07-03T06:58:17Z
dc.date.available 2018-07-03T06:58:17Z
dc.date.issued 2018-06-28
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/105086
dc.description.abstract [EN] Skin is an important trait for Rex rabbits and skin development is influenced by many processes, including hair follicle cycling, keratinocyte differentiation and formation of coat colour and skin morphogenesis. We identified differentially expressed microRNAs (miRNAs) between the back and belly skin in Rex rabbits. In total, 211 miRNAs (90 upregulated miRNAs and 121 downregulated miRNAs) were identified with a |log2 (fold change)|>1 and P-value<0.05. Using target gene prediction for the miRNAs, differentially expressed predicted target genes were identified and the functional enrichment and signalling pathways of these target genes were processed to reveal their biological functions. A number of differentially expressed miRNAs were found to be involved in regulation of the cell cycle, skin epithelium differentiation, keratinocyte proliferation, hair follicle development and melanogenesis. In addition, target genes regulated by miRNAs play key roles in the activities of the Hedgehog signalling pathway, Wnt signalling pathway, Osteoclast differentiation and MAPK pathway, revealing mechanisms of skin development. Nine candidate miRNAs and 5 predicted target genes were selected for verification of their expression by quantitative reverse transcription polymerase chain reaction. A regulation network of miRNA and their target genes was constructed by analysing the GO enrichment and signalling pathways. Further studies should be carried out to validate the regulatory relationships between candidate miRNAs and their target genes. es_ES
dc.description.sponsorship This study was supported by the Modern Agricultural Industrial System Special Funding (CARS-44-A-1), the Priority Academic Programme Development of Jiangsu Higher Education Institutions (2014-134) and the General Programme of Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (16KJB230001). es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Rex rabbit es_ES
dc.subject Back skin es_ES
dc.subject Belly skin es_ES
dc.subject miRNA es_ES
dc.subject Transcriptome es_ES
dc.title Identification and profiling of microRNA between back and belly Skin in Rex rabbits (Oryctolagus cuniculus) es_ES
dc.type Artículo es_ES
dc.date.updated 2018-06-29T09:57:22Z
dc.identifier.doi 10.4995/wrs.2018.7058
dc.relation.projectID info:eu-repo/grantAgreement/Natural Science Foundation of Jiangsu Province//16KJB230001/
dc.relation.projectID info:eu-repo/grantAgreement/PAPD//2014-134/
dc.relation.projectID info:eu-repo/grantAgreement/Earmarked Fund for Modern Agro-industry Technology Research System, China//CARS-44-A-1/
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Zhao, B.; Chen, Y.; Mu, L.; Hu, S.; Wu, X. (2018). Identification and profiling of microRNA between back and belly Skin in Rex rabbits (Oryctolagus cuniculus). World Rabbit Science. 26(2):179-190. https://doi.org/10.4995/wrs.2018.7058 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2018.7058 es_ES
dc.description.upvformatpinicio 179 es_ES
dc.description.upvformatpfin 190 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26
dc.description.issue 2
dc.identifier.eissn 1989-8886
dc.contributor.funder Natural Science Research of Jiangsu Higher Education Institutions of China
dc.contributor.funder Priority Academic Program Development of Jiangsu Higher Education Institutions
dc.contributor.funder Earmarked Fund for Modern Agro-industry Technology Research System, China
dc.contributor.funder Natural Science Foundation of Jiangsu Province es_ES
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