Corominas, J.; Ramayo-Caldas, Y.; Castelló, A.; Muñoz, M.; Ibáñez-Escriche, N.; Folch, JM.; Ballester, M. (2012). Evaluation of the porcine ACSL4 gene as a candidate gene for meat quality traits in pigs. Animal Genetics. 43(6):714-720. https://doi.org/10.1111/j.1365-2052.2012.02335.x
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/138903
Title:
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Evaluation of the porcine ACSL4 gene as a candidate gene for meat quality traits in pigs
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Author:
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Corominas, J.
Ramayo-Caldas, Y.
Castelló, A.
Muñoz, M.
Ibáñez-Escriche, Noelia
Folch, J. M.
Ballester, M.
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UPV Unit:
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Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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[EN] Long-chain acyl-CoA synthetase (ACSL) family members catalyse the formation of long-chain acyl-CoA from fatty acid, ATP and CoA, thus playing an important role in both de novo lipid synthesis and fatty acid catabolism. ...[+]
[EN] Long-chain acyl-CoA synthetase (ACSL) family members catalyse the formation of long-chain acyl-CoA from fatty acid, ATP and CoA, thus playing an important role in both de novo lipid synthesis and fatty acid catabolism. Previous studies in our group evaluated ACSL4 as a positional candidate gene for quantitative trait loci located on chromosome X in an Iberian x Landrace cross. A DQ144454:c.2645G>A SNP located in the 3' untranslated region of the ACSL4 gene was associated with the percentages of oleic and monounsaturated fatty acids. The aim of the present work was to evaluate the functional implication of this genetic variant. An expression analysis was performed for 120 individuals with different genotypes for the DQ144454:c.2645G>A polymorphism using real-time quantitative PCR. Differences between genotypes were identified in liver, with the ACSL4 mRNA expression levels higher in animals with the G allele than in animals with the A allele. A SNP genome-wide association study with ACSL4 relative expression levels showed significant positions on chromosomes 6 and 12. Description of positional candidate genes for ACSL4 regulation on chromosomes 6 and 12 is provided.
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Subjects:
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ACSL4
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Fatty acid metabolism
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Genome-wide association study
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Ss-oxidation
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Copyrigths:
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Cerrado |
Source:
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Animal Genetics. (issn:
0268-9146
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DOI:
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10.1111/j.1365-2052.2012.02335.x
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Publisher:
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Blackwell Publishing
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Publisher version:
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https://doi.org/10.1111/j.1365-2052.2012.02335.x
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Project ID:
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MECD/AP2008-01450
MICINN/AGL2008-04818-C03
MECD/BES-2009-018223
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Thanks:
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We would like to thank Dr. Jordi Estelle for help in the association studies for SSCX SNPs and Dr. Anna Mercade for the genotyping of porcine chip. This study has been funded by MICINN project (AGL2008-04818-C03). J. ...[+]
We would like to thank Dr. Jordi Estelle for help in the association studies for SSCX SNPs and Dr. Anna Mercade for the genotyping of porcine chip. This study has been funded by MICINN project (AGL2008-04818-C03). J. Corominas was funded by a FPI PhD grant from the Spanish Ministerio de Educacion (BES-2009-018223) and Y. Ramayo by a FPU PhD grant (AP2008-01450).
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Type:
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Artículo
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