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Fibrinogen organization at the cell-material interface directs endothelial cell behavior

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Fibrinogen organization at the cell-material interface directs endothelial cell behavior

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dc.contributor.author Gugutkov, Dencho es_ES
dc.contributor.author González García, Cristina es_ES
dc.contributor.author Altankov, George es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2020-04-17T12:49:40Z
dc.date.available 2020-04-17T12:49:40Z
dc.date.issued 2011 es_ES
dc.identifier.issn 0883-9115 es_ES
dc.identifier.uri http://hdl.handle.net/10251/140890
dc.description.abstract [EN] Fibrinogen (FG) adsorption on surfaces with controlled fraction of -OH groups was investigated with AFM and correlated to the initial interaction of primary endothelial cells (HUVEC). The -OH content was tailored making use of a family of copolymers consisting of ethyl acrylate (EA) and hydroxyl ethyl acrylate (HEA) in different ratios. The supramolecular distribution of FG changed from an organized network-like structure on the most hydrophobic surface (-OH 0) to dispersed molecular aggregate one as the fraction of -OH groups increases, indicating a different conformation by the adsorbed protein. The best cellular interaction was observed on the most hydrophobic (-OH 0) surface where FG assembled in a fibrin-like appearance in the absence of any thrombin. Likewise, focal adhesion formation and actin cytoskeleton development was poorer as the fraction of hydroxy groups on the surface was increased. The biological activity of the surface-induced FG network to provide 3D cues in a potential tissue engineered scaffold, making use of electrospun PEA fibers (-OH 0), seeded with human umbilical vein endothelial cells was investigated. The FG assembled on the polymer fibers gave rise to a biologically active network able to direct cell orientation along the fibers (random or aligned), promote cytoskeleton organization and focal adhesion formation. © 2011 The Author(s). es_ES
dc.description.sponsorship AFM was performed under the technical guidance of the Microscopy Service at the Universidad Politecnica de Valencia, whose advice is greatly appreciated. The work was supported by the Spanish Ministry of Science and Innovation through projects nos MAT2009-14440-C02-01, MAT2009-14440-C02-02 and EULANEST PIM2010EEU-00111. es_ES
dc.language Inglés es_ES
dc.publisher SAGE Publications es_ES
dc.relation MICINN/MAT2009-14440-C02-02 es_ES
dc.relation MICINN/EULANEST PIM2010EEU-00111 es_ES
dc.relation MINISTERIO DE EDUCACION /MAT2009-14440-C02-01 es_ES
dc.relation.ispartof Journal of Bioactive and Compatible Polymers es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cell-material interactions es_ES
dc.subject Cell-material interface es_ES
dc.subject Electrospun fibers es_ES
dc.subject Endothelial cell behavior es_ES
dc.subject Ethyl acrylate es_ES
dc.subject Fibrinogen es_ES
dc.subject Fibrinogen organization es_ES
dc.subject HUVEC es_ES
dc.subject Hydroxyl ethyl acrylate es_ES
dc.subject Cell-material interaction es_ES
dc.subject Ethyl acrylates es_ES
dc.subject Adhesion es_ES
dc.subject Adsorption es_ES
dc.subject Electrospinning es_ES
dc.subject Fibers es_ES
dc.subject Hydrophobicity es_ES
dc.subject Interfaces (materials) es_ES
dc.subject Pressure effects es_ES
dc.subject Proteins es_ES
dc.subject Scaffolds (biology) es_ES
dc.subject Surface chemistry es_ES
dc.subject Surfaces es_ES
dc.subject Tissue es_ES
dc.subject Endothelial cells es_ES
dc.subject Acrylic acid 2 hydroxyethyl ester es_ES
dc.subject Acrylic acid derivative es_ES
dc.subject Acrylic acid ethyl ester es_ES
dc.subject Biomaterial es_ES
dc.subject Unclassified drug es_ES
dc.subject Actin filament es_ES
dc.subject Article es_ES
dc.subject Atomic force microscopy es_ES
dc.subject Cell adhesion es_ES
dc.subject Controlled study es_ES
dc.subject Endothelium cell es_ES
dc.subject Human es_ES
dc.subject Human cell es_ES
dc.subject Protein analysis es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Fibrinogen organization at the cell-material interface directs endothelial cell behavior es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1177/0883911511409020 es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Gugutkov, D.; González García, C.; Altankov, G.; Salmerón Sánchez, M. (2011). Fibrinogen organization at the cell-material interface directs endothelial cell behavior. Journal of Bioactive and Compatible Polymers. 26(4):375-387. https://doi.org/10.1177/0883911511409020 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1177/0883911511409020 es_ES
dc.description.upvformatpinicio 375 es_ES
dc.description.upvformatpfin 387 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26 es_ES
dc.description.issue 4 es_ES
dc.relation.pasarela S\211756 es_ES
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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