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Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase

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Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase

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Morin, J.; Cao, F.; Lázaro, J.; Arias-Gonzalez, JR.; Valpuesta, J.; Carrascosa, J.; Salas, M.... (2015). Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase. Nucleic Acids Research. 43(7):3643-3652. https://doi.org/10.1093/nar/gkv204

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Title: Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase
Author: Morin, J.A. Cao, F.J. Lázaro, J.M. Arias-Gonzalez, J. R. Valpuesta, J.M. Carrascosa, J.L. Salas, M. Ibarra, B.
UPV Unit: Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada
Issued date:
Abstract:
[EN] During DNA replication replicative polymerases move in discrete mechanical steps along the DNA template. To address how the chemical cycle is coupled to mechanical motion of the enzyme, here we use optical tweezers ...[+]
Subjects: DNA replication , DNA polymerase , Single-molecule , Mechano-chemistry
Copyrigths: Reconocimiento (by)
Source:
Nucleic Acids Research. (issn: 0305-1048 )
DOI: 10.1093/nar/gkv204
Publisher:
Oxford University Press
Publisher version: https://doi.org/10.1093/nar/gkv204
Project ID:
MINECO/BFU2011-29038
...[+]
MINECO/BFU2011-29038
MINECO/BFU2013-44202
MINECO/BFU2011-23645
MINECO/FIS2010-17440
MINECO/GR35/10-A920
MINECO/GR35/10-A911
MINECO/MAT2013-49455-EXP
MINECO/BFU2012-31825
MINECO/CDS2007-0015
EMBO/ASTF 276-2012
CAM/S2009/MAT/1507
[-]
Thanks:
We thank Stephan Grill laboratory (MPI-CBG, Dresden) for help with data collection and E. Galburt, M. Manosas and M. De Vega for critical reading of the manuscript. Spanish Ministry of Economy and Competitiveness [BFU2011-29038 ...[+]
Type: Artículo

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