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Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase

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Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase

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dc.contributor.author Morin, J.A. es_ES
dc.contributor.author Cao, F.J. es_ES
dc.contributor.author Lázaro, J.M. es_ES
dc.contributor.author Arias-Gonzalez, J. R. es_ES
dc.contributor.author Valpuesta, J.M. es_ES
dc.contributor.author Carrascosa, J.L. es_ES
dc.contributor.author Salas, M. es_ES
dc.contributor.author Ibarra, B. es_ES
dc.date.accessioned 2020-10-22T03:32:07Z
dc.date.available 2020-10-22T03:32:07Z
dc.date.issued 2015-04-20 es_ES
dc.identifier.issn 0305-1048 es_ES
dc.identifier.uri http://hdl.handle.net/10251/152802
dc.description.abstract [EN] During DNA replication replicative polymerases move in discrete mechanical steps along the DNA template. To address how the chemical cycle is coupled to mechanical motion of the enzyme, here we use optical tweezers to study the translocation mechanism of individual bacteriophage Phi29 DNA polymerases during processive DNA replication. We determine the main kinetic parameters of the nucleotide incorporation cycle and their dependence on external load and nucleotide (dNTP) concentration. The data is inconsistent with power stroke models for translocation, instead supports a loose-coupling mechanism between chemical catalysis and mechanical translocation during DNA replication. According to this mechanism the DNA polymerase works by alternating between a dNTP/PPi-free state, which diffuses thermally between pre- and post-translocated states, and a dNTP/PPi-bound state where dNTP binding stabilizes the post-translocated state. We show how this thermal ratchet mechanism is used by the polymerase to generate work against large opposing loads (~50 pN). es_ES
dc.description.sponsorship We thank Stephan Grill laboratory (MPI-CBG, Dresden) for help with data collection and E. Galburt, M. Manosas and M. De Vega for critical reading of the manuscript. Spanish Ministry of Economy and Competitiveness [BFU2011-29038 to J.L.C., BFU2013-44202 to J.M.V., BFU2011-23645 to M.S., FIS2010-17440, GR35/10-A920GR35/10-A-911 to F.J.C., MAT2013-49455-EXP to J.R.A.-G. and BFU2012-31825 to B.I.]; Regional Government of Madrid [S2009/MAT 1507 to J.L.C. and CDS2007-0015 to M.S.]; European Molecular Biology Organization [ASTF 276-2012 to J.M.L.]. Funding for open access charge: Spanish Ministry of Economy and Competitiveness [BFU2012-31825 to B.I.]. es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Nucleic Acids Research es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject DNA replication es_ES
dc.subject DNA polymerase es_ES
dc.subject Single-molecule es_ES
dc.subject Mechano-chemistry es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/nar/gkv204 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-29038-C02-02/ES/ANALISIS ESTRUCTURAL INTEGRADO A DISTINTOS NIVELES DE RESOLUCION: ESTUDIO DE SISTEMAS VIRALES MODELO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2013-44202-P/ES/CHAPERONAS MOLECULARES: UNA CADENA DE MONTAJE DE MAQUINAS MOLECULARES INVOLUCRADA EN EL PLEGAMIENTO Y DEGRADACION DE PROTEINAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-23645/ES/REPLICACION DEL DNA DE Ø29 INICIADA CON PROTEINA TERMINAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//FIS2010-17440/ES/FISICA DE LOS PROCESOS FUERA DEL EQUILIBRIO: RETROALIMENTACION, ENTROPIA E INFORMACION/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//GR35%2F10-A920/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//GR35%2F10-A911/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2013-49455-EXP/ES/G-CUADRUPLEX COMO INTERRUPTOR MOLECULAR CONTROLADO POR NANOPARTICULAS Y DEMOSTRADO POR PINZAS OPTICAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-31825/ES/DINAMICA DE LA REPLICACION DEL ADN MITOCONDRIAL A NIVEL DE MOLECULAS INDIVIDUALES./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//CSD2007-00015/ES/INESTABILIDAD GENOMICA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EMBO//ASTF 276-2012/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Gobierno de la Comunidad de Madrid//S2009%2FMAT-1507/ES/Nuevos materiales y dispositivos biofuncionales híbridos en nanociencia/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Morin, J.; Cao, F.; Lázaro, J.; Arias-Gonzalez, JR.; Valpuesta, J.; Carrascosa, J.; Salas, M.... (2015). Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase. Nucleic Acids Research. 43(7):3643-3652. https://doi.org/10.1093/nar/gkv204 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/nar/gkv204 es_ES
dc.description.upvformatpinicio 3643 es_ES
dc.description.upvformatpfin 3652 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 43 es_ES
dc.description.issue 7 es_ES
dc.identifier.pmid 25800740 es_ES
dc.identifier.pmcid PMC4402526 es_ES
dc.relation.pasarela S\407995 es_ES
dc.contributor.funder Comunidad de Madrid es_ES
dc.contributor.funder European Molecular Biology Organization es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Ministerio de Ciencia e Innovación
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