Comparative Studies on the Physiological and Biochemical Responses to Salt Stress of Eggplant (Solanum melongena) and Its Rootstock S. torvum

Abstract

[EN] This study investigated the physiological and biochemical responses to salinity stress of Solanum melongena and its wild relative, Solanum torvum, commonly used as eggplant rootstock. Young plants of both species were watered during 25 days with NaCl aqueous solutions at the following four final concentrations: 0 (for the controls), 100, 200, and 300 mM. Plant growth parameters, photosynthetic pigments content, monovalent ion concentrations in roots and leaves, leaf levels of osmolytes (proline and total soluble sugars), oxidative stress markers (MDA and H2O2), non-enzymatic antioxidants (total phenolic compounds and total flavonoids), and enzymatic antioxidant activities superoxide dismutase, catalase, glutathione reductase) were determined after the stress treatments. Salt-induced growth reduction was more significant in S. melongena than in S. torvum, especially at high salt concentrations, indicating a (slightly) higher salt tolerance of the wild species. The mechanisms of tolerance of S. torvum were partly based on the active transport of toxic ions to the leaves at high external salinity and, presumably, a better capacity to store them in the vacuoles, as well as on the accumulation of proline to higher concentrations than in the cultivated eggplant. MDA and H2O2 contents did not vary in response to the salt treatments in S. torvum. However, in S. melongena, MDA content increased by 78% when 300 mM NaCl was applied. No activation of antioxidant mechanisms, accumulation of antioxidant compounds, or increase in the specific activity of antioxidant enzymes in any of the studied species was induced by salinity. The relatively high salt tolerance of S. torvum supports its use as rootstock for eggplant cultivation in salinized soils and as a possible source of salt-tolerance genes for the genetic improvement of cultivated eggplant.This study investigated the physiological and biochemical responses to salinity stress of Solanum melongena and its wild relative, Solanum torvum, commonly used as eggplant rootstock. Young plants of both species were watered during 25 days with NaCl aqueous solutions at the following four final concentrations: 0 (for the controls), 100, 200, and 300 mM. Plant growth parameters, photosynthetic pigments content, monovalent ion concentrations in roots and leaves, leaf levels of osmolytes (proline and total soluble sugars), oxidative stress markers (MDA and H2O2), non-enzymatic antioxidants (total phenolic compounds and total flavonoids), and enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione reductase) were determined after the stress treatments. Salt-induced growth reduction was more significant in S. melongena than in S. torvum, especially at high salt concentrations, indicating a (slightly) higher salt tolerance of the wild species. The mechanisms of tolerance of S. torvum were partly based on the active transport of toxic ions to the leaves at high external salinity and, presumably, a better capacity to store them in the vacuoles, as well as on the accumulation of proline to higher concentrations than in the cultivated eggplant. MDA and H2O2 contents did not vary in response to the salt treatments in S. torvum. However, in S. melongena, MDA content increased by 78% when 300 mM NaCl was applied. No activation of antioxidant mechanisms, accumulation of antioxidant compounds, or increase in the specific activity of antioxidant enzymes in any of the studied species was induced by salinity. The relatively high salt tolerance of S. torvum supports its use as rootstock for eggplant cultivation in salinized soils and as a possible source of salt-tolerance genes for the genetic improvement of cultivated eggplant.