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dc.contributor.author | Sanza, F.J. | es_ES |
dc.contributor.author | Holgado, M. | es_ES |
dc.contributor.author | Ortega Higueruelo, Francisco José | es_ES |
dc.contributor.author | Casquel Del Campo, Rafael | es_ES |
dc.contributor.author | López-Romero, D. | es_ES |
dc.contributor.author | Bañuls Polo, Mª José | es_ES |
dc.contributor.author | Laguna, Mari Fe | es_ES |
dc.contributor.author | Barrios, C.A. | es_ES |
dc.contributor.author | Puchades Pla, Rosa | es_ES |
dc.contributor.author | Maquieira Catala, Ángel | es_ES |
dc.date.accessioned | 2015-01-15T10:57:49Z | |
dc.date.available | 2015-01-15T10:57:49Z | |
dc.date.issued | 2011 | es_ES |
dc.identifier.issn | 0956-5663 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/46093 | |
dc.description.abstract | [EN] In a previous work we introduced the term Bio-Photonic Sensing Cells (BICELLs), referred to periodic networks of nano-pillar suitable for biosensing when are vertically interrogated. In this article, we demonstrate the biosensing capabilities of a type of micrometric size BICELLs made of SU-8 nano-pillars fabricated over transparent substrates. We verify the biochips functionality comparing the theoretical simulations with the experimental results when are optically interrogated in transmission. We also demonstrate a sensitivity enhancement by reducing the pitch among nano-pillars from 800 to 700. nm. Thus, the Limit of Detection achievable in these types of BICELLs is in the order of 64. pg/mL for 700. nm in pitch among nano-pillars in comparison with 292. pg/mL for 800. nm in pitch when are interrogated by Fourier Transform Visible and Infrared Spectrometry. The experiments exhibited a good reproducibility with a relative standard deviation of 0.29% measured within 8 days for a specific concentration. Finally, BICELLs functionality was tested in real conditions with unpurified rabbit serum for detecting anti-gestrinone antibodies, demonstrating the high performance of this type of BICELLs to detect specific antibodies having immobilized the suitable bioreceptors onto the sensing surface. © 2011 Elsevier B.V. | es_ES |
dc.description.sponsorship | This work is done within the support of the Spanish Ministry of Science and Innovation under project BIOPSIA (REF: TEC2008-06574). The authors thanks the Comunidad de Madrid and Universidad Politecncia de Madrid (Project BIO-VERSATIL, Ref CCG10-UPM/SEM-5096), Generalitat Valenciana (project ACOMP/2010/009 and PROMETEO 2010/008) and Dr Eva Brun for providing the rabbit serum containing polyclonal antibodies for gestrinone studies. F.J.O. is grateful to the Generalitat Valenciana for the postdoctoral grant included in the VALi + d 2010 Programme for Postdoctoral Researchers. | en_EN |
dc.language | Inglés | |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Biosensors and Bioelectronics | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Anti-gestrinone detection | es_ES |
dc.subject | Optical biosensing | es_ES |
dc.subject | SU-8 nano-pillar | es_ES |
dc.subject | Biophotonic sensing | es_ES |
dc.subject | Bioreceptors | es_ES |
dc.subject | Biosensing | es_ES |
dc.subject | Biosensing capability | es_ES |
dc.subject | Infrared spectrometry | es_ES |
dc.subject | Limit of detection | es_ES |
dc.subject | Optical bio-sensing | es_ES |
dc.subject | Relative standard deviations | es_ES |
dc.subject | Reproducibilities | es_ES |
dc.subject | Sensing surface | es_ES |
dc.subject | Sensitivity enhancements | es_ES |
dc.subject | Specific concentration | es_ES |
dc.subject | Theoretical simulation | es_ES |
dc.subject | Transparent substrate | es_ES |
dc.subject | Antibodies | es_ES |
dc.subject | Bioassay | es_ES |
dc.subject | Biochips | es_ES |
dc.subject | Fourier transforms | es_ES |
dc.subject | Infrared spectroscopy | es_ES |
dc.subject | Chemical detection | es_ES |
dc.subject | Gestrinone | es_ES |
dc.subject | Article | es_ES |
dc.subject | Bio Photonic Sensing Cell | es_ES |
dc.subject | Biochip | es_ES |
dc.subject | Biofilm | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject | Comparative study | es_ES |
dc.subject | Controlled study | es_ES |
dc.subject | Electric field | es_ES |
dc.subject | Immunoassay | es_ES |
dc.subject | Process optimization | es_ES |
dc.subject | Reproducibility | es_ES |
dc.subject | Sensitivity analysis | es_ES |
dc.subject | Simulation | es_ES |
dc.subject | Thickness | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Biosensing Techniques | es_ES |
dc.subject | Equipment Design | es_ES |
dc.subject | Nanostructures | es_ES |
dc.subject | Optics and Photonics | es_ES |
dc.subject | Polymers | es_ES |
dc.subject | Rabbits | es_ES |
dc.subject | Oryctolagus cuniculus | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing | |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.bios.2011.06.010 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//TEC2008-06574-C03-03/ES/DESARROLLO DE ESTRUCTURAS NANO-OPTO-FLUIDICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//TEC2008-06574-C03-02/ES/ESTRUCTURAS NANO-OPTO-FLUIDICAS PARA SENSADO BIOQUIMICO BASADO EN TECNICAS DE INTERROGACION OPTICA DE ALTA SENSIBILIDAD/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPM//CCG10-UPM%2FSEM-5096/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//TEC2008-06574-C03-01/ES/ESTRUCTURAS NANO-OPTO-FLUIDICAS PARA SENSADO BIOQUIMICO BASADO EN TECNICAS DE INTERROGACION OPTICA DE ALTA SENSIBILIDAD/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACOMP%2F2010%2F009/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/ES/AYUDA PARA EL GRUPO SYM-IDM/ | |
dc.rights.accessRights | Cerrado | |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Sanza, F.; Holgado, M.; Ortega Higueruelo, FJ.; Casquel Del Campo, R.; López-Romero, D.; Bañuls Polo, MJ.; Laguna, MF.... (2011). Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing. Biosensors and Bioelectronics. 26:4842-4847. https://doi.org/10.1016/j.bios.2011.06.010 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.description.upvformatpinicio | 4842 | es_ES |
dc.description.upvformatpfin | 4847 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 26 | es_ES |
dc.relation.senia | 212793 | es_ES |
dc.identifier.pmid | 21733669 | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | |
dc.contributor.funder | Universidad Politécnica de Madrid | |
dc.contributor.funder | Generalitat Valenciana |