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A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato

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A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato

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dc.contributor.author Lisón Párraga, María Purificación es_ES
dc.contributor.author Tarraga Herrero, Susana es_ES
dc.contributor.author López Gresa, Mª Pilar es_ES
dc.contributor.author Sauri Ferrando, Asunción es_ES
dc.contributor.author Torres Vidal, Cristina es_ES
dc.contributor.author Campos Beneyto, Laura es_ES
dc.contributor.author Belles Albert, José Mª es_ES
dc.contributor.author Conejero Tomás, Vicente es_ES
dc.contributor.author Rodrigo Bravo, Ismael es_ES
dc.date.accessioned 2016-03-14T14:02:47Z
dc.date.issued 2013-03
dc.identifier.issn 1615-9853
dc.identifier.uri http://hdl.handle.net/10251/61835
dc.description This is the accepted version of the following article: Lisón, P., Tárraga, S., López-Gresa, P., Saurí, A., Torres, C., Campos, L., Bellés, J. M., Conejero, V. and Rodrigo, I. (2013), A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato. Proteomics, 13: 833–844, which has been published in final form at http://dx.doi.org/10.1002/pmic.201200286. es_ES
dc.description.abstract Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected by Citrus exocortis viroid (CEVd). Among the differentially expressed proteins detected, 45 were successfully identified and classified into different functional categories. Validation results by RT-PCR allowed us to classify the proteins into two expression groups. First group included genes with changes at the transcriptional level upon CEVd infection, such as an endochitinase, a β-glucanase, and pathogenesis-related proteins, PR10 and P69G. All these defense proteins were also induced by gentisic acid, a pathogen-induced signal in compatible interactions. The second group of proteins showed no changes at the transcriptional level and included several ribosomal proteins and translation factors, such as the elongation factors 1 and 2 and the translation initiation factor 5-alpha. These results were validated by 2D Western blot, and possible PTMs caused by CEVd infection were detected. Moreover, an interaction between eukaryotic elongation factor 1 and CEVd was observed by 2D Northwestern. The present study provides new protein-related information on the mechanisms of plant resistance to pathogens. es_ES
dc.description.sponsorship We would like to thank the Proteomic Service of the IBMCP (Instituto de Biologia Molecular y Celular de Plantas, Valencia, Spain) for the technical assistance. We also thank Dr. Alejandro Ferrando (Instituto de Biologia Molecular y Celular de Plantas UPV-CSIC) for critical reading of the manuscript, discussions, and for kindly providing us with the eIF5A antisera. We are also grateful to Dr. Brenda Hunter (University of Arizona) for both eEF1A and eEF2 antiserum. This work was supported by Grant BFU2009-11958 from Direccion General de Programas y Transferencia de Conocimiento, from Spanish Ministry of Science and Innovation. Laura Campos was the recipient of a predoctoral fellowship ACIF/2010/231 from Generalitat Valenciana (Spain). M. Pilar Lopez-Gresa held a postdoctoral fellowship JAEDoc_08_ 00402 from the Consejo Superior de Investigaciones Cientificas (Spain). en_EN
dc.language Inglés es_ES
dc.publisher Wiley-Blackwell es_ES
dc.relation.ispartof Proteomics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject 2D DIGE es_ES
dc.subject Plant stress es_ES
dc.subject Tomato es_ES
dc.subject Translation factors es_ES
dc.subject Viroid es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/pmic.201200286
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2009-11958/ES/Señalizacion Y Respuesta Defensiva De Las Plantas Frente A Patogenos/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2010%2F231/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CSIC//JAEDoc 08 00402/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Lisón Párraga, MP.; Tarraga Herrero, S.; López Gresa, MP.; Sauri Ferrando, A.; Torres Vidal, C.; Campos Beneyto, L.; Belles Albert, JM.... (2013). A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato. Proteomics. 13(5):833-844. https://doi.org/10.1002/pmic.201200286 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1002/pmic.201200286 es_ES
dc.description.upvformatpinicio 833 es_ES
dc.description.upvformatpfin 844 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 253922 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
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