Soler-Botija, C.; Bago, JR.; Llucia-Valldeperas, A.; Vallés Lluch, A.; Castells-Sala, C.; Martinez-Ramos, C.; Fernandez-Muinos, T.... (2014). Engineered 3D bioimplants using elastomeric scaffold, self-assembling peptide hydrogel, and adipose tissue-derived progenitor cells for cardiac regeneration. American Journal of Translational Research. 6:291-301. http://hdl.handle.net/10251/63949
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/63949
Título:
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Engineered 3D bioimplants using elastomeric scaffold, self-assembling peptide hydrogel, and adipose tissue-derived progenitor cells for cardiac regeneration
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Autor:
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Soler-Botija, Carolina
Bago, Juli R.
Llucia-Valldeperas, Aida
Vallés Lluch, Ana
Castells-Sala, Cristina
Martinez-Ramos, Cristina
Fernandez-Muinos, Teresa
Chachques, Juan Carlos
Monleón Pradas, Manuel
Semino, Carlos E.
Bayes-Genis, Antoni
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Entidad UPV:
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Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular
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Fecha difusión:
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Resumen:
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[EN] Contractile restoration of myocardial scars remains a challenge with important clinical implications. Here, a combination of porous elastomeric membrane, peptide hydrogel, and subcutaneous adipose tissue-derived ...[+]
[EN] Contractile restoration of myocardial scars remains a challenge with important clinical implications. Here, a combination of porous elastomeric membrane, peptide hydrogel, and subcutaneous adipose tissue-derived progenitor cells (subATDPCs) was designed and evaluated as a bioimplant for cardiac regeneration in a mouse model of myocardial infarction. SubATDPCs were doubly transduced with lentiviral vectors to express bioluminescent-fluorescent reporters driven by constitutively active, cardiac tissue-specific promoters. Cells were seeded into an engineered bioimplant consisting of a scaffold (polycaprolactone methacryloyloxyethyl ester) filled with a peptide hydrogel (PuraMatrix(TM)), and transplanted to cover injured myocardium. Bioluminescence and fluorescence quantifications showed de novo and progressive increases in promoter expression in bioactive implant-treated animals. The bioactive implant was well adapted to the heart, and fully functional vessels traversed the myocardium-bioactive implant interface. Treatment translated into a detectable positive effect on cardiac function, as revealed by echocardiography. Thus, this novel implant is a promising construct for supporting myocardial regeneration.
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Palabras clave:
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Cardiac regeneration
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Subcutaneous ATDPCs
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Self-assembling peptide hydrogel
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Elastomeric membrane
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RECATABI
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Derechos de uso:
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Reconocimiento - No comercial (by-nc)
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Fuente:
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American Journal of Translational Research. (issn:
1943-8141
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Editorial:
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e-Century Publishing
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Versión del editor:
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http://www.ajtr.org/
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Código del Proyecto:
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info:eu-repo/grantAgreement/MICINN//SAF2011-30067-C02-01/ES/DESARROLLO DE BIOIMPLANTES PARA LA REGENERACION CARDIACA CON CELULAS PROGENITORAS DERIVADAS DE LA GRASA CARDIACA, BIOMATERIALES Y MONITORIZACION EN LINEA: EL "SMART PATCH"/
info:eu-repo/grantAgreement/EC/FP7/229239/EU/Regeneration of Cardiac Tissue Assisted by Bioactive Implants/
info:eu-repo/grantAgreement/Fundació La Marató de TV3//080330/
info:eu-repo/grantAgreement/MINECO//RD12%2F0019%2F0029/ES/Terapia Celular/
info:eu-repo/grantAgreement/MINECO//RD12%2F0042%2F0047/ES/Enfermedades cardiovasculares/
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Agradecimientos:
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The research leading to these results received funding from the European Union Seventh Framework Programme (Project RECATABI,
7FP/2007-2013) under grant agreement number 229239. This work was also supported by Ministerio ...[+]
The research leading to these results received funding from the European Union Seventh Framework Programme (Project RECATABI,
7FP/2007-2013) under grant agreement number 229239. This work was also supported by Ministerio de Ciencia e Innovación (SAF2011-
30067-C02-01), Fundació La Marató de TV3 (080330), Red de Terapia Celular-TerCel (RD12/0019/0029), Red Cardio-vascular
(RD12/0042/0047), Sociedad Española de Cardiología, and Fundació Privada Daniel Bravo Andreu
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Tipo:
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Artículo
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