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dc.contributor.author | Gamboa Martínez, Tatiana Carolina | es_ES |
dc.contributor.author | Gómez Ribelles, José Luís | es_ES |
dc.contributor.author | Gallego Ferrer, Gloria | es_ES |
dc.date.accessioned | 2016-05-17T07:12:21Z | |
dc.date.available | 2016-05-17T07:12:21Z | |
dc.date.issued | 2011-09 | |
dc.identifier.issn | 0883-9115 | |
dc.identifier.uri | http://hdl.handle.net/10251/64167 | |
dc.description.abstract | A hybrid scaffold was obtained by the deposition of a thin network of submicron fibrin fibrils on the microporous walls of a macroporous poly(L-lactide) (PLLA) three-dimensional structure. The fibrin coating is homogeneous across the entire substrate and allowed the pore structure remain open in the hybrid scaffold. The elastic modulus of the hybrid scaffold (0.65 MPa) was increased up to twofold compared to the pure PLLA scaffold (0.29 MPa). Mouse pre-osteoblastic cells, MC3T3, were seeded on both pure PLLA and hybrid scaffolds, and cultured for 3, 6, and 24 h. The coating enhanced the cell colonization and proliferation and provided a more homogeneous distribution of cells within the scaffolds. In addition, the coating improved the scaffold adhesion properties by supplying new binding sites to the cells that modify the transmembrane receptors involved in initial cell adhesion mechanism. The expression of the ß3 integrin was observed in cells cultured on fibrin-coated scaffolds instead of the ?5 integrin, which was expressed in the uncoated scaffold. These hybrid PLLA/fibrin scaffolds have cell culture features suitable to promote early tissue regeneration. | es_ES |
dc.description.sponsorship | The authors acknowledge the financial support of the Spanish Ministry through the DPI2007-65601-C03-03 and HP2007-0103 projects. T. Gamboa Martinez is grateful to the Centro de Investigacion Principe Felipe for the assistance in the use of the CLSM. G. Gallego Ferrer and J. L. Gomez Ribelles acknowledge the support by funds for research in the field of Regenerative Medicine through the collaboration agreement from the Conselleria de Sanidad (Generalitat Valenciana), and the Instituto de Salud Carlos III (Ministry of Science and Innovation). | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | SAGE Publications (UK and US) | es_ES |
dc.relation.ispartof | Journal of Bioactive and Compatible Polymers | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Cell adhesion | es_ES |
dc.subject | Coating | es_ES |
dc.subject | Fibrin | es_ES |
dc.subject | Scaffolds | es_ES |
dc.subject | Tissue engineering | es_ES |
dc.subject | Adhesion mechanisms | es_ES |
dc.subject | Adhesion properties | es_ES |
dc.subject | Cell colonization | es_ES |
dc.subject | Homogeneous distribution | es_ES |
dc.subject | Hybrid scaffolds | es_ES |
dc.subject | Integrins | es_ES |
dc.subject | Macroporous | es_ES |
dc.subject | Microporous walls | es_ES |
dc.subject | PLLA | es_ES |
dc.subject | Poly-L-lactide | es_ES |
dc.subject | Scaffolds for tissue engineering | es_ES |
dc.subject | Submicron | es_ES |
dc.subject | Three-dimensional structure | es_ES |
dc.subject | Tissue regeneration | es_ES |
dc.subject | Transmembrane receptors | es_ES |
dc.subject | Adhesion | es_ES |
dc.subject | Binding sites | es_ES |
dc.subject | Cell culture | es_ES |
dc.subject | Cells | es_ES |
dc.subject | Coatings | es_ES |
dc.subject | Mammals | es_ES |
dc.subject | Tissue | es_ES |
dc.subject | Scaffolds (biology) | es_ES |
dc.subject | Beta3 integrin | es_ES |
dc.subject | Membrane receptor | es_ES |
dc.subject | Poly(levo lactide) | es_ES |
dc.subject | Tissue scaffold | es_ES |
dc.subject | Unclassified drug | es_ES |
dc.subject | Article | es_ES |
dc.subject | Binding site | es_ES |
dc.subject | Cell proliferation | es_ES |
dc.subject | Immunofluorescence test | es_ES |
dc.subject | Osteoblast | es_ES |
dc.subject | Protein expression | es_ES |
dc.subject | Scanning electron microscopy | es_ES |
dc.subject | Stress strain relationship | es_ES |
dc.subject.classification | MAQUINAS Y MOTORES TERMICOS | es_ES |
dc.title | Fibrin coating on poly (L-lactide) scaffolds for tissue engineering | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1177/0883911511419834 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//DPI2007-65601-C03-03/ES/DISEÑO DE NUEVOS CONSTRUCTOS POLIMERICOS BIODEGRADABLES PARA LA REGENRACION OSTEOCONDRAL/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//HP2007-0103/ES/HP2007-0103/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada | es_ES |
dc.description.bibliographicCitation | Gamboa Martínez, TC.; Gómez Ribelles, JL.; Gallego Ferrer, G. (2011). Fibrin coating on poly (L-lactide) scaffolds for tissue engineering. Journal of Bioactive and Compatible Polymers. 26(5):464-477. https://doi.org/10.1177/0883911511419834 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1177/0883911511419834 | es_ES |
dc.description.upvformatpinicio | 464 | es_ES |
dc.description.upvformatpfin | 477 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 26 | es_ES |
dc.description.issue | 5 | es_ES |
dc.relation.senia | 211288 | es_ES |
dc.identifier.eissn | 1530-8030 | |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
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