Arouri, R.; Le Goff, G.; Hemden, H.; Navarro-Llopis, V.; M'saad, M.; Castanera, P.; Feyereisen, R.... (2015). Resistance to lambda-cyhalothrin in Spanish field populations of Ceratitis capitata and metabolic resistance mediated by P450 in a resistant strain. Pest Management Science. 71(9):1281-1291. doi:10.1002/ps.3924
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/64703
Title:
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Resistance to lambda-cyhalothrin in Spanish field populations of Ceratitis capitata and metabolic resistance mediated by P450 in a resistant strain
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Author:
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Arouri, Rabeh
Le Goff, Gaelle
Hemden, Hiethem
Navarro-Llopis, Vicente
M'saad, Mariem
Castanera, Pedro
Feyereisen, Rene
Hernandez-Crespo, Pedro
Ortego, Felix
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UPV Unit:
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Universitat Politècnica de València. Centro de Ecología Química Agrícola - Centre d'Ecologia Química Agrícola
Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani
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Issued date:
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Abstract:
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BACKGROUND
The withdrawal of malathion in the European Union in 2009 resulted in a large increase in lambda-cyhalothrin applications for the control of the Mediterranean fruit fly, Ceratitis capitata, in Spanish citrus ...[+]
BACKGROUND
The withdrawal of malathion in the European Union in 2009 resulted in a large increase in lambda-cyhalothrin applications for the control of the Mediterranean fruit fly, Ceratitis capitata, in Spanish citrus crops.
RESULTS
Spanish field populations of C. capitata have developed resistance to lambda-cyhalothrin (6 14-fold), achieving LC50 values (129 287 ppm) higher than the recommended concentration for field treatments (125 ppm). These results contrast with the high susceptibility to lambda-cyhalothrin found in three Tunisian field populations. We have studied the mechanism of resistance in the laboratory-selected resistant strain W-1Kλ (205-fold resistance). Bioassays with synergists showed that resistance was almost completely suppressed by the P450 inhibitor PBO. The study of the expression of 53 P450 genes belonging to the CYP4, CYP6, CYP9 and CYP12 families in C. capitata revealed that CYP6A51 was overexpressed (13 18-fold) in the resistant strain. The W-1Kλ strain also showed high levels of cross-resistance to etofenprox (240-fold) and deltamethrin (150-fold).
CONCLUSION
Field-evolved resistance to lambda-cyhalothrin has been found in C. capitata. Metabolic resistance mediated by P450 appears to be the main resistance mechanism in the resistant strain W-1Kλ. The levels of cross-resistance found may compromise the effectiveness of other pyrethroids for the control of this species.
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Subjects:
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Fruit fly
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Pyrethroid
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Insecticide resistance
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P450 overexpression
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Copyrigths:
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Reconocimiento - No comercial (by-nc)
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Source:
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Pest Management Science. (issn:
1526-498X
)
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DOI:
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10.1002/ps.3924
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Publisher:
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Wiley
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Publisher version:
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http://dx.doi.org/10.1002/ps.3924
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Project ID:
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USDA-APHIS
...[+]
USDA-APHIS
USDA-ARS
NHGRI
CICYT AGL2010-21349-C02-01/AGR
AECID A/026050/09
AECID A/030253/10
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Thanks:
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We gratefully acknowledge the Medfly Whole Genome Sequencing Project for providing the NCBI sequence data used in this report, led by Drs Alfred Handler (USDA, Agricultural Research Service), Giuliano Gasperi (University ...[+]
We gratefully acknowledge the Medfly Whole Genome Sequencing Project for providing the NCBI sequence data used in this report, led by Drs Alfred Handler (USDA, Agricultural Research Service), Giuliano Gasperi (University of Pavia) and Stephen Richards (Baylor College of Medicine), and supported by funding from USDA-APHIS, USDA-ARS and NHGRI; Rafael Argiles (TRAGSA, Valencia), Ramon Tora (Unitat Sanitat Vegetal del DAR, Lleida) and Emilio Guirado (IHSM-CSIC, Malaga) for assistance in field sampling; and Maria Torne (Dow Agro-Science Iberica) and Stephen Skillman (Syngenta AG) for providing technical-grade spinosad and lufenuron respectively. This work received financial support from CICYT (AGL2010-21349-C02-01/AGR) and AECID (A/026050/09 and A/030253/10).
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Type:
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Artículo
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