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Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus

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Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus

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dc.contributor.author Lalic, Jasna es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2016-10-31T12:38:33Z
dc.date.available 2016-10-31T12:38:33Z
dc.date.issued 2012-08
dc.identifier.issn 0018-067X
dc.identifier.uri http://hdl.handle.net/10251/72998
dc.description.abstract How epistatic interactions between mutations determine the genetic architecture of fitness is of central importance in evolution. The study of epistasis is particularly interesting for RNA viruses because of their genomic compactness, lack of genetic redundancy, and apparent low complexity. Moreover, interactions between mutations in viral genomes determine traits such as resistance to antiviral drugs, virulence and host range. In this study we generated 53 Tobacco etch potyvirus genotypes carrying pairs of single-nucleotide substitutions and measured their separated and combined deleterious fitness effects. We found that up to 38% of pairs had significant epistasis for fitness, including both positive and negative deviations from the null hypothesis of multiplicative effects. Interestingly, the sign of epistasis was correlated with viral protein-protein interactions in a model network, being predominantly positive between linked pairs of proteins and negative between unlinked ones. Furthermore, 55% of significant interactions were cases of reciprocal sign epistasis (RSE), indicating that adaptive landscapes for RNA viruses maybe highly rugged. Finally, we found that the magnitude of epistasis correlated negatively with the average effect of mutations. Overall, our results are in good agreement to those previously reported for other viruses and further consolidate the view that positive epistasis is the norm for small and compact genomes that lack genetic robustness. Heredity (2012) 109, 71-77; doi: 10.1038/hdy.2012.15; published online 11 April 2012 es_ES
dc.description.sponsorship We thank Francisca de la Iglesia and Angels Prosper for their excellent technical assistance, Stephanie Bedhomme and Mark P Zwart for the discussion and Mario A Fares for statistical advice. Jose A Daros generously gifted us the pMTEV plasmid. This research was supported by the Spanish Ministry of Science and Innovation grant BFU2009-06993 to SFE. JL was supported by the JAE program from CSIC. en_EN
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Heredity es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Epistasis es_ES
dc.subject Fitness landscapes es_ES
dc.subject Genome architecture es_ES
dc.subject Virus evolution es_ES
dc.title Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/hdy.2012.15
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2009-06993/ES/Biologia Evolutiva Y De Sistemas De La Emergencia De Fitovirus De Rna/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Lalic, J.; Elena Fito, SF. (2012). Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus. Heredity. 109(2):71-77. https://doi.org/10.1038/hdy.2012.15 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/hdy.2012.15 es_ES
dc.description.upvformatpinicio 71 es_ES
dc.description.upvformatpfin 77 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 109 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 232201 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
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