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Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production.

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Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production.

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dc.contributor.author Rambla Nebot, Jose Luis es_ES
dc.contributor.author Trapero-Mozos, Almudena es_ES
dc.contributor.author Diretto, Gianfranco es_ES
dc.contributor.author Rubio-Moraga, Angela es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Gomez-Gomez, Lourdes es_ES
dc.contributor.author Ahrazem, Oussama es_ES
dc.date.accessioned 2017-05-15T10:34:33Z
dc.date.available 2017-05-15T10:34:33Z
dc.date.issued 2016-10-27
dc.identifier.issn 1664-462X
dc.identifier.uri http://hdl.handle.net/10251/81129
dc.description.abstract [EN] Volatile compounds are the major determinants of aroma and flavor in both grapes and wine. In this study, we investigated the emission of volatile and non-volatile compounds during berry maturation in two grape varieties (Airen and Tempranillo) throughout 2010 and 2011. HS-SPME coupled to gas chromatography and mass spectrometry was applied for the identification and relative quantitation of these compounds. Principal component analysis was performed to search for variability between the two cultivars and evolution during 10 developmental stages. Results showed that there are distinct differences in volatile compounds between cultivars throughout fruit development. Early stages were characterized in both cultivars by higher levels of some apocarotenoids such as beta-cyclocitral or beta-ionone, terpenoids (E)-linalool oxide and (Z)-linalool oxide and several furans, while the final stages were characterized by the highest amounts of ethanol, benzenoid phenylacetaldehyde and 2-phenylethanol, branched-amino acid-derived 3-methylbutanol and 2-methylbutanol, and a large number of lipid derivatives. Additionally, we measured the levels of the different classes of volatile precursors by using liquid chromatography coupled to high resolution mass spectrometry. In both varieties, higher levels of carotenoid compounds were detected in the earlier stages, zeaxanthin and alpha-carotene were only detected in Airen while neoxanthin was found only in Tempranillo; more variable trends were observed in the case of the other volatile precursors. Furthermore, we monitored the expression of homolog genes of a set of transcripts potentially involved in the biosynthesis of these metabolites, such as some glycosyl hydrolases family 1, lipoxygenases, alcohol dehydrogenases hydroperoxide lyases, O-methyltransferases and carotenoid cleavage dioxygenases during the defined developmental stages. Finally, based on Pearson correlation analyses, we explored the metabolite-metabolite fluctuations within VOCs/precursors during the berry development; as well as tentatively linking the formation of some metabolites detected to the expression of some of these genes. Our data showed that the two varieties displayed a very different pattern of relationships regarding the precursor/volatile metabolite-metabolite fluctuations, being the lipid and the carotenoid metabolism the most distinctive between the two varieties. Correlation analysis showed a higher degree of overall correlation in precursor/volatile metabolite-metabolite levels in Airen, confirming the enriched aroma bouquet characteristic of the white varieties. es_ES
dc.description.sponsorship We thank J. Argandona (Institute Botanico, Universidad de Castilla-La Mancha, Albacete, Spain) for excellent technical support, and K.A. Walsh for language revision. This work was supported by the "Junta de comunidades de Castilla-La Mancha" (JCCM) [PPII10-0062-7718] and benefited from the networking activities within the European Cooperation in Science and Technology Action CA15136 (EUROCAROTEN). GD was supported by short-term fellowships of the Quality Fruit (FA1106) European Cooperation in Science and Technology actions. OA was funded by FPCYTCLM through the INCRECYT Programme.
dc.language Inglés es_ES
dc.publisher Frontiers Media es_ES
dc.relation JCCM/PPII10-0062-7718 es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Volatile organic compounds es_ES
dc.subject Precursors es_ES
dc.subject Aroma es_ES
dc.subject Expression analysis es_ES
dc.subject Vitis vinifera es_ES
dc.title Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2016.01619
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Rambla Nebot, JL.; Trapero-Mozos, A.; Diretto, G.; Rubio-Moraga, A.; Granell Richart, A.; Gomez-Gomez, L.; Ahrazem, O. (2016). Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production. Frontiers in Plant Science. 7(1619):1-23. doi:10.3389/fpls.2016.01619 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3389/fpls.2016.01619 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 23 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 1619 es_ES
dc.relation.senia 331626 es_ES
dc.identifier.pmid 27833635 en_EN
dc.identifier.pmcid PMC5082229 en_EN
dc.contributor.funder Junta de Comunidades de Castilla-La Mancha


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