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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Arrillaga, I.; Guevara, MA.; Muñoz Bertomeu, J.; Lazaro-Gimeno, D.; Saez-Laguna, E.; Diaz, L.; Torralba, L.... (2014). Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species. Plant Cell, Tissue and Organ Culture. 118(1):147-155. doi:10.1007/s11240-014-0470-z

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Título: Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species
Autor:
Entidad UPV: Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Fecha difusión:
Resumen:
[EN] The potential of haploid tissues for genetic studies in conifers is hampered by the lack of abundant and homogeneous plant material suitable for DNA isolation. In this work we have determined factors promoting haploid ...[+]
Palabras clave: Chromosome counting , Flow cytometry , Genome sequencing , Haploid callus , Pinus pinaster
Derechos de uso: Cerrado
Fuente:
Plant Cell, Tissue and Organ Culture. (issn: 0167-6857 ) (eissn: 1573-5044 )
DOI: 10.1007/s11240-014-0470-z
Editorial:
Springer Verlag (Germany)
Versión del editor: http://doi.org/10.1007/s11240-014-0470-z
Código del Proyecto: info:eu-repo/grantAgreement/EC/FP7/289841/EU
Tipo: Artículo

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