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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Arrillaga, I.; Guevara, MA.; Muñoz Bertomeu, J.; Lazaro-Gimeno, D.; Saez-Laguna, E.; Diaz, L.; Torralba, L.... (2014). Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species. Plant Cell, Tissue and Organ Culture. 118(1):147-155. doi:10.1007/s11240-014-0470-z

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Title: Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species
Author:
UPV Unit: Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Issued date:
Abstract:
[EN] The potential of haploid tissues for genetic studies in conifers is hampered by the lack of abundant and homogeneous plant material suitable for DNA isolation. In this work we have determined factors promoting haploid ...[+]
Subjects: Chromosome counting , Flow cytometry , Genome sequencing , Haploid callus , Pinus pinaster
Copyrigths: Cerrado
Source:
Plant Cell, Tissue and Organ Culture. (issn: 0167-6857 ) (eissn: 1573-5044 )
DOI: 10.1007/s11240-014-0470-z
Publisher:
Springer Verlag (Germany)
Publisher version: http://doi.org/10.1007/s11240-014-0470-z
Project ID: info:eu-repo/grantAgreement/EC/FP7/289841/EU
Thanks:
Research funds were provided by Spanish National projects (BIO2007-29814 and BIO2010-12302-E) and Generalitat Valenciana (Prometeo/2009/075). The research leading to these results has also received funding from the European ...[+]
Type: Artículo

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