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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Arrillaga, I.; Guevara, MA.; Muñoz Bertomeu, J.; Lazaro-Gimeno, D.; Saez-Laguna, E.; Diaz, L.; Torralba, L.... (2014). Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species. Plant Cell, Tissue and Organ Culture. 118(1):147-155. https://doi.org/10.1007/s11240-014-0470-z

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Título: Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species
Autor: Arrillaga, Isabel Guevara, María Angeles Muñoz Bertomeu, Jesús Lazaro-Gimeno, David Saez-Laguna, Enrique Diaz, Luis-Manuel Torralba, L Mendoza-Poudereux, Isabel Segura Garcia del Rio, Juan Cervera, María Teresa
Entidad UPV: Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Fecha difusión:
Resumen:
[EN] The potential of haploid tissues for genetic studies in conifers is hampered by the lack of abundant and homogeneous plant material suitable for DNA isolation. In this work we have determined factors promoting haploid ...[+]
Palabras clave: Chromosome counting , Flow cytometry , Genome sequencing , Haploid callus , Pinus pinaster
Derechos de uso: Cerrado
Fuente:
Plant Cell, Tissue and Organ Culture. (issn: 0167-6857 ) (eissn: 1573-5044 )
DOI: 10.1007/s11240-014-0470-z
Editorial:
Springer Verlag (Germany)
Versión del editor: http://doi.org/10.1007/s11240-014-0470-z
Código del Proyecto:
info:eu-repo/grantAgreement/EC/FP7/289841/EU/Promoting a functional and comparative understanding of the conifer genome- implementing applied aspects for more productive and adapted forests./
info:eu-repo/grantAgreement/MEC//BIO2007-29814-E/ES/PARTICIPACION EN LA PRIMERA FASE DE LA INICIATIVA INTERNACIONAL PARA LA SECUENCIACION DEL GENOMA DE PINO/
info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F075/
info:eu-repo/grantAgreement/MICINN//BIO2010-12302-E/ES/ESTUDIO DEL GENOMA DE PINUS PINASTER: BASES PARA IMPLEMENTAR LA PRODUCTIVIDAD Y ADAPTACION DE ESPECIES FORESTALES/
Agradecimientos:
Research funds were provided by Spanish National projects (BIO2007-29814 and BIO2010-12302-E) and Generalitat Valenciana (Prometeo/2009/075). The research leading to these results has also received funding from the European ...[+]
Tipo: Artículo

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