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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

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Nombre: Isabel Arrillaga; ...
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dc.contributor.author Arrillaga, Isabel es_ES
dc.contributor.author Guevara, María Angeles es_ES
dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Lazaro-Gimeno, David es_ES
dc.contributor.author Saez-Laguna, Enrique es_ES
dc.contributor.author Diaz, Luis-Manuel es_ES
dc.contributor.author Torralba, L es_ES
dc.contributor.author Mendoza-Poudereux, Isabel es_ES
dc.contributor.author Segura Garcia del Rio, Juan es_ES
dc.contributor.author Cervera, María Teresa es_ES
dc.date.accessioned 2017-07-19T09:26:55Z
dc.date.available 2017-07-19T09:26:55Z
dc.date.issued 2014-07
dc.identifier.issn 0167-6857
dc.identifier.uri http://hdl.handle.net/10251/85437
dc.description.abstract [EN] The potential of haploid tissues for genetic studies in conifers is hampered by the lack of abundant and homogeneous plant material suitable for DNA isolation. In this work we have determined factors promoting haploid callus induction and proliferation from megagametophytes of Oria 6, a genotype of Pinus pinaster Aiton (maritime pine) from the natural population Sierra de Oria (Almeria, Spain), selected based on its response to extreme drought conditions. The generation of haploid callus was restricted to megagametophytes isolated from light-brown cones with no dehydrated seeds collected in September. Culture medium composition did not significantly affect callus induction, but a modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine favored further multiplication. The ploidy status of the callus lines was determined by flow cytometry and seven polymorphic microsatellites. A total of sixteen haploid callus lines were established and one of these is being used as a source of DNA for massive sequencing of maritime pine genome. es_ES
dc.description.sponsorship Research funds were provided by Spanish National projects (BIO2007-29814 and BIO2010-12302-E) and Generalitat Valenciana (Prometeo/2009/075). The research leading to these results has also received funding from the European Union’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreement No. 289841 (ProCoGen). We thank M. A. Morcillo for technical assistance, Dr. Jose´ Juarez (Instituto Valenciano de Investigaciones Agrarias, IVIA, Spain) and Dr. Vesselina Nikolova (Vegetable Crops Research Institute, Plovdiv, Bulgaria) for their support in the flow cytometry and karyological analyses, respectively.
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Plant Cell, Tissue and Organ Culture es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Chromosome counting es_ES
dc.subject Flow cytometry es_ES
dc.subject Genome sequencing es_ES
dc.subject Haploid callus es_ES
dc.subject Pinus pinaster es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11240-014-0470-z
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/289841/EU/Promoting a functional and comparative understanding of the conifer genome- implementing applied aspects for more productive and adapted forests./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BIO2007-29814-E/ES/PARTICIPACION EN LA PRIMERA FASE DE LA INICIATIVA INTERNACIONAL PARA LA SECUENCIACION DEL GENOMA DE PINO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F075/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2010-12302-E/ES/ESTUDIO DEL GENOMA DE PINUS PINASTER: BASES PARA IMPLEMENTAR LA PRODUCTIVIDAD Y ADAPTACION DE ESPECIES FORESTALES/
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Arrillaga, I.; Guevara, MA.; Muñoz Bertomeu, J.; Lazaro-Gimeno, D.; Saez-Laguna, E.; Diaz, L.; Torralba, L.... (2014). Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species. Plant Cell, Tissue and Organ Culture. 118(1):147-155. https://doi.org/10.1007/s11240-014-0470-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s11240-014-0470-z es_ES
dc.description.upvformatpinicio 147 es_ES
dc.description.upvformatpfin 155 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 118 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 270133 es_ES
dc.identifier.eissn 1573-5044
dc.contributor.funder European Commission
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
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