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Ultrastructural immunolocalization of arabinogalactan protein, pectin and hemicellulose epitopes through anther development in Brassica napus

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Ultrastructural immunolocalization of arabinogalactan protein, pectin and hemicellulose epitopes through anther development in Brassica napus

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dc.contributor.author Corral Martínez, Patricia es_ES
dc.contributor.author García-Fortea, Edgar es_ES
dc.contributor.author Bernard, Sophie es_ES
dc.contributor.author Driouich, Azeddine es_ES
dc.contributor.author Seguí-Simarro, Jose M. es_ES
dc.date.accessioned 2018-02-06T08:19:55Z
dc.date.available 2018-02-06T08:19:55Z
dc.date.issued 2016 es_ES
dc.identifier.issn 0032-0781 es_ES
dc.identifier.uri http://hdl.handle.net/10251/96982
dc.description.abstract [EN] In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development. We used immunogold labeling to study the spatial and temporal patterns of composition and distribution of different AGP, pectin, xyloglucan and xylan epitopes in high pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages. We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan. Each cell wall epitope showed a characteristic temporal and spatial labeling pattern. Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns. Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development. The AGP epitopes studied appeared to belong to AGPs specifically involved in microspore differentiation, and contributed first by the tapetum and then, upon tapetal dismantling, by the endothecium and middle layers. In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture. The distribution of the different cell wall constituents is regulated in a tissue and stage-specific manner, which seems directly related with the role of each tissue at each stage. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministry of Economy (MINECO) jointly funded by le Fonds europeen de developpement regional (FEDER) [grants AGL2010-17895, AGL2014-55177 to J.M.S.S.]; La Region de Haute Normandie and le Grand Reseau de Recherche-Vegetal, Agronomie, Sol et Innovation, l'Universite de Rouen [financial support to A.D.]; FEDER [financial support to A.D.]; l'Agence nationale de la recherche (ANR) [financial support to A.D.]. en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Plant and Cell Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject AGPs es_ES
dc.subject Cell wall es_ES
dc.subject Microspore es_ES
dc.subject Pollen es_ES
dc.subject Xylan es_ES
dc.subject Xyloglucan es_ES
dc.subject Electron Microscopy Service of the UPV
dc.subject.classification GENETICA es_ES
dc.title Ultrastructural immunolocalization of arabinogalactan protein, pectin and hemicellulose epitopes through anther development in Brassica napus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/pcp/pcw133 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-55177-R/ES/NUEVAS VIAS DE MEJORA DE LA EMBRIOGENESIS DE MICROSPORAS EN SOLANACEAS RECALCITRANTES: ESTUDIO DE LA AUTOFAGIA, LA UPR Y LA REGULACION HORMONAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2010-17895/ES/GENERACION EFICIENTE DE DOBLE HAPLOIDES EN BERENJENA Y PIMIENTO MEDIANTE CULTIVO IN VITRO DE MICROSPORAS AISLADAS. ANALISIS CELULAR Y MOLECULAR DEL DESARROLLO ANDROGENICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Corral Martínez, P.; García-Fortea, E.; Bernard, S.; Driouich, A.; Seguí-Simarro, JM. (2016). Ultrastructural immunolocalization of arabinogalactan protein, pectin and hemicellulose epitopes through anther development in Brassica napus. Plant and Cell Physiology. 57(10):2161-2174. https://doi.org/10.1093/pcp/pcw133 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1093/pcp/pcw133 es_ES
dc.description.upvformatpinicio 2161 es_ES
dc.description.upvformatpfin 2174 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 57 es_ES
dc.description.issue 10 es_ES
dc.identifier.pmid 27481894
dc.relation.pasarela S\318748 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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