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Browsing by Subject "ELISA"

Now showing items 1-20 of 20

A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla

Mazzeo ., Ilaria; Giorgini, E.; Gioacchini, G.; Maradonna, F.; Vilchez Olivencia, Maria Carmen; Baloche, S.; Dufour, S.; Pérez Igualada, Luz María; Carnevali, O.; Asturiano Nemesio, Juan Francisco (Blackwell Publishing, 2016)

[EN] A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic ...
A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection

Montoya Baides, Ángel; March Iborra, Mª Del Carmen; Montagut Ferizzola, Yeison Javier; Moreno Tamarit, Mª José; Manclus Ciscar, Juan José; Arnau Vives, Antonio; Jiménez Jiménez, Yolanda; Jaramillo, Marisol; Marin, Paula A.; Torres-Villa, Róbinson Alberto (Bentham Science Publishers, 2017)

Tuberculosis, one of the oldest diseases affecting human beings, is still considered as a world public health problem by the World Health Organization. Therefore, there is a need for new and more powerful analytical methods ...
Comparative Analysis of Chromatin-Delivered Biomarkers in the Monitoring of Sepsis and Septic Shock: A Pilot Study

Beltrán-García, Jesús; Manclus Ciscar, Juan José; García-López, Eva M.; Carbonell, Nieves; Ferreres, José; Rodríguez-Gimillo, María; Garcés, Concepción; Pallardó, Federico V.; García-Giménez, José L.; Montoya, Ángel; Romá-Mateo, Carlos (MDPI AG, 2021-09)

[EN] Sepsis management remains one of the most important challenges in modern clinical practice. Rapid progression from sepsis to septic shock is practically unpredictable, hence the critical need for sepsis biomarkers ...
Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit juices

Moreno Tamarit, Mª José; Plana Andani, Emma; Manclus Ciscar, Juan José; Montoya Baides, Ángel (Springer Verlag (Germany), 2014-02)

[EN] A comparative study of the analytical performance of enzyme-linked immunosorbent assays (ELISAs), based on monoclonal and recombinant antibodies, for the determination of fungicide residues in fruit juices has been ...
Detection of chemical residues in tangerine juices by a duplex immunoassay

Navarro Martínez, Patricia; Pérez, A.J.; Gabaldón, José Antonio; Núñez-Delicado, E.; Puchades, Rosa; Maquieira Catala, Ángel; Morais, Sergi (Elsevier, 2013-11-15)

[EN] A rapid duplex ELISA for the simultaneous determination of two of the most widely used organophosphorous insecticides in tangerine juices is described. To accomplish this aim, two individual enzyme-linked immunosorbent ...
Determinación de la proteína C reactiva en especies de animales con diferentes patologías

Samper Avilés, Marta (Universitat Politècnica de València, 2015-10-15)

[ES] Las enfermedades que cursan con un proceso inflamatorio (infección, trauma, heridas¿) están asociadas a signos fisiológicos característicos y, a cambios en ciertas proteínas séricas, conocidas como proteínas de fase ...
Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices

Plana Andani, Emma; Moreno Tamarit, Mª José; Montoya Baides, Ángel; Manclus Ciscar, Juan José (Elsevier, 2014-01-15)

Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in ...
Development of monoclonal antibody-based immunoassays for the analysis of bisphenol A in canned vegetables

Moreno Tamarit, Mª José; D'Arienzo, Pasquale; Manclus Ciscar, Juan José; Montoya Baides, Ángel (2011)

The aim of this work was the development of monoclonal antibodies (MAbs) and highly sensitive immunoassays (ELISAs) to bisphenol A (BPA), a well-known endocrine disruptor able to migrate from the internal coating of cans ...
Evaluación de la técnica basada en inmunoensayo con microesferas magnéticas (Luminex®) para medición simultánea de varias citoquinas en plasma humano, y comparativa con la metodología estándar (ELISA)

Pardo Sánchez, José Miguel (Universitat Politècnica de València, 2014-07-31)

[EN] Immunoassays are normally used in order to measure cytokines in patients samples. Among them, ELISA is the standard technique, though the novel Luminex® xMAP® technology is more suitable for the new molecular biology ...
Humoral immune response to different routes of myxomatosis vaccine application

Manev, I.; Genova, K.; Lavazza, A.; Capucci, L. (Universitat Politècnica de València, 2018-06-28)

[EN] The aim of our study was to monitor the dynamics of the serological response to different application routes of live attenuated myxomatosis vaccine. The study included 42 Californian breed rabbits, aged 3 mo, of both ...
Massive Immuno Multiresidue Screening of Water Pollutants

Dobosz, Paulina Dorota; Morais, Sergi; Bonet, Emilio; Puchades, Rosa; Maquieira Catala, Ángel (American Chemical Society, 2015-10-06)

[EN] An immuno multiresidue screening assay in microarray format for the determination of complex chemical mixtures at the microgram per liter level, using antibody-functionalized gold nanoparticles, is presented. The ...
Multiresidue determination of antibiotics in feed and fish samples for food safety evaluation. Comparison of immunoassay vs LC-MS-MS

Cháfer Pericás, María Consuelo; Maquieira Catala, Ángel; Puchades, Rosa; Miralles, Javier; Moreno, Amelia (Elsevier, 2011-06)

[EN] Antibiotic residues (sulfonamides and tetracyclines) were determined in Gilthead sea bream (Sparus aurata) and feed samples by means of immunoassays and LC-MS-MS (liquid chromatography-mass spectrometry 2). A preliminary ...
Occurrence of aflatoxin M1 in the Manchego cheese supply chain

Rubio R.; Licón C.; Berruga Fernandez, Maria Isabel; Molina Pons, Mª Pilar; Molina Casanova, Ana (American Dairy Science Association, 2011)

The importance of ewe milk lies in the production of high quality cheeses, such as Manchego cheese with a Protected Designation of Origin, whose safety must be guaranteed. In a 2-yr study, 407 bulk tank milk samples from ...
Puesta a punto del protocolo de cribado por resistencia al virus del amarilleo de las cucurbitáceas transmitido por pulgones (CABYV) en cucurbitáceas

Sánchez Hernández, Francisco José (Universitat Politècnica de València, 2022-10-18)

[ES] La familia cucurbitaceae presenta una gran importancia económica. Entre las especies de esta familia destacan la sandía (Citrullus lanatus (Thunb.) Matsum. & Nakai.), el pepino (Cucumis sativus L.), el calabacín y la ...
Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis

Santiago Felipe, Sara; Tortajada-Genaro, Luis Antonio; Puchades, Rosa; Maquieira Catala, Ángel (Elsevier, 2014-02-06)

[EN] Polymerase chain reaction in conjunction with enzyme-linked immunosorbent assay (PCR-ELISA) is a well-established technique that provides a suitable rapid, sensitive, and selective method for a broad range of applications. ...
Seed transmission of Melon necrotic spot virus and efficacy of seed-disinfection treatments.

Herrera-Vásquez, J. A.; Córdoba-Sellés, M. C.; Cebrián, M. C.; Alfaro Fernández, Ana Olvido; Jordá, C. (Blackwell Publishing, 2009-05-14)

[EN] Rates of seed transmission of Melon necrotic spot virus (MNSV) were estimated in seedlings grown from commercial melon (Cucumis melo) cv. Galia F1 seeds. Seedlings at the cotyledon stage and adult plants were assayed ...
Seroprevalence and Prevalence of Infectious Bronchitis Virus in broilers, laying hens and broiler breeders in Spain

Cortés, Verónica; Sevilla-Navarro, Sandra; García, Cristina; Marín, Clara; Catalá-Gregori, Pablo (Oxford University Press, 2022-05)

[EN] Infectious Bronchitis Virus (IBV) is one of the most important viral diseases who causes important economic losses in poultry industry. This study aimed to assess the seroprevalence, prevalence, and variants of IBV ...
Simultaneous detection and identification of Pepino mosaic virus (PepMV) isolates by multiplex one-step RT-PCR

Alfaro Fernández, Ana Olvido; SANCHEZ NAVARRO, JESUS ANGEL; Cebrián, María del Carmen; Córdoba-Sellés, Maria del Carmen; Pallás Benet, Vicente; Jordá, Concepción (Springer-Verlag, 2009-09)

[EN] A RT-PCR was developed for the simultaneous detection and identification of three groups of Pepino mosaic virus (PepMV): European/Peruvian, Chilean 1/US1 and Chilean 2/US2 groups, followed by a restriction analysis ...
Synthesis and screening of selective alpha-v-beta-6 integrin antagonists

Valle Ribera, Guillermo Samuel del (Universitat Politècnica de València, 2023-09-13)

[EN] Idiopathic pulmonary fibrosis (IPF) is a progressive, chronic respiratory disorder marked by excessive lung tissue scarring. Integrin αVß6 is a crucial molecular player in the onset of pulmonary fibrosis. For different ...
Turnip yellow mosaic virus in Chinese cabbage in Spain: Commercial seed transmission and molecular characterization

Alfaro Fernández, Ana Olvido; Serrano, A.; Tornos, Teodora; Cebrian Mico, Maria Carmen; Córdoba-Sellés, Maria del Carmen; Jordá, Concepción; Font San Ambrosio, Maria Isabel (2016)

[EN] Seed transmission of Turnip yellow mosaic virus (TYMV, genus Tymovirus) was evaluated in the whole seeds and seedlings that emerged from three commercial Chinese cabbage (Brassica pekinensis) seed batches. Seedlings ...