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Role of material-driven fibronectin fibrillogenesis in cell differentiation

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Role of material-driven fibronectin fibrillogenesis in cell differentiation

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dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Moratal Pérez, David es_ES
dc.contributor.author Lee, Ted T. es_ES
dc.contributor.author Schwarzbauer, Jean E. es_ES
dc.contributor.author Garcia, Andres J. es_ES
dc.date.accessioned 2013-10-31T13:44:30Z
dc.date.issued 2011
dc.identifier.issn 0142-9612
dc.identifier.uri http://hdl.handle.net/10251/33174
dc.description.abstract Fibronectin (FN) is a ubiquitous extracellular matrix protein (ECM) protein that is organized into fibrillar networks by cells through an integrin-mediated process that involves contractile forces. This assembly allows for the unfolding of the FN molecule, exposing cryptic domains that are not available in the native globular FN structure and activating intracellular signalling complexes. However, organization of FN into a physiological fibrillar network upon adsorption on a material surface has not been observed. Here we demonstrate cell-free, material-induced FN fibrillogenesis into a biological matrix with enhanced cellular activities. We found that simple FN adsorption onto poly(ethyl acrylate) surfaces, but not control polymers, triggered FN organization into a fibrillar network via interactions in the amino-terminal 70 kDa fragment, which is involved in the formation of cell-mediated FN fibrils. Moreover, the material-driven FN fibrils exhibited enhanced biological activities in terms of myogenic differentiation compared to individual FN molecules and even type I collagen. Our results demonstrate that molecular assembly of FN can take place at the material interface, giving rise to a physiological protein network similar to fibrillar matrices assembled by cells. This research identifies material surfaces that trigger the organization of extracellular matrix proteins into biological active fibrils and establishes a new paradigm to engineer ECM-mimetic biomaterials. es_ES
dc.description.sponsorship This work was supported by NSF DMR-0909002 and MAT2009-14440-C02-01. MSS was supported by the Spanish Government through PR2009-0351 to stay in Atlanta (Georgia Institute of Technology) for a sabbatical during 2010. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation NSF [DMR-0909002, MAT2009-14440-C02-01] es_ES
dc.relation Spanish Government [PR2009-0351] es_ES
dc.relation.ispartof Biomaterials es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cell adhesion es_ES
dc.subject Fibronectin es_ES
dc.subject Integrin es_ES
dc.subject Interface es_ES
dc.subject Biological activities es_ES
dc.subject Biological matrixes es_ES
dc.subject Cell-free es_ES
dc.subject Cellular activities es_ES
dc.subject Contractile force es_ES
dc.subject Extracellular matrix protein es_ES
dc.subject Fibrillar networks es_ES
dc.subject Fibrillogenesis es_ES
dc.subject In-cell es_ES
dc.subject Integrins es_ES
dc.subject Intracellular signalling es_ES
dc.subject Material interfaces es_ES
dc.subject Material surface es_ES
dc.subject Molecular assembly es_ES
dc.subject Poly (ethyl acrylate) es_ES
dc.subject Protein network es_ES
dc.subject Type I collagen es_ES
dc.subject Adhesion es_ES
dc.subject Adsorption es_ES
dc.subject Complexation es_ES
dc.subject Enzyme activity es_ES
dc.subject Materials es_ES
dc.subject Molecular interactions es_ES
dc.subject Physiology es_ES
dc.subject Surface properties es_ES
dc.subject Tissue es_ES
dc.subject Proteins es_ES
dc.subject Acrylic acid ethyl ester es_ES
dc.subject Biomaterial es_ES
dc.subject Collagen fibril es_ES
dc.subject Collagen type 1 es_ES
dc.subject Polymer es_ES
dc.subject Amino terminal sequence es_ES
dc.subject Animal cell es_ES
dc.subject Article es_ES
dc.subject Biological activity es_ES
dc.subject Cell activity es_ES
dc.subject Cell differentiation es_ES
dc.subject Controlled study es_ES
dc.subject Muscle cell es_ES
dc.subject Muscle development es_ES
dc.subject Nonhuman es_ES
dc.subject Priority journal es_ES
dc.subject Protein assembly es_ES
dc.subject Protein protein interaction es_ES
dc.subject Acrylic Resins es_ES
dc.subject Animals es_ES
dc.subject Biocompatible Materials es_ES
dc.subject Bone Cements es_ES
dc.subject Cell Line es_ES
dc.subject Extracellular Matrix es_ES
dc.subject Fibronectins es_ES
dc.subject Focal Adhesion Protein-Tyrosine Kinases es_ES
dc.subject Humans es_ES
dc.subject Materials Testing es_ES
dc.subject Mice es_ES
dc.subject Microscopy, Atomic Force es_ES
dc.subject Polymethyl Methacrylate es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Role of material-driven fibronectin fibrillogenesis in cell differentiation es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1016/j.biomaterials.2010.11.057
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Salmerón Sánchez, M.; Rico Tortosa, PM.; Moratal Pérez, D.; Lee, TT.; Schwarzbauer, JE.; Garcia, AJ. (2011). Role of material-driven fibronectin fibrillogenesis in cell differentiation. Biomaterials. 32(8):2099-2105. doi:10.1016/j.biomaterials.2010.11.057 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.biomaterials.2010.11.057 es_ES
dc.description.upvformatpinicio 2099 es_ES
dc.description.upvformatpfin 2105 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 32 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 212173


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