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Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR

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Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR

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Moreno Trigos, MY.; Ballesteros, L.; García Hernández, J.; Santiago Cuéllar, P.; González Pellicer, A.; Ferrús Pérez, MA. (2011). Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR. Water Research. 45(15):4634-4640. https://doi.org/10.1016/j.watres.2011.06.015

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Title: Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR
Author: Moreno Trigos, Mª Yolanda Ballesteros, Lorena García Hernández, Jorge Santiago Cuéllar, Paula González Pellicer, Ana Ferrús Pérez, Mª Antonia
UPV Unit: Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Issued date:
Abstract:
Listeria monocytogenes detection in wastewater can be difficult because of the large amount of background microbiota and the presence of viable but non-culturable forms in this environment. The aim of this study was to ...[+]
Subjects: Listeria monocytogenes , PCR , Wastewater , Wastewater samples , Wastewater treatment plants , Assays , Cell culture , Diseases , Fluorescence , Listeria , Oligonucleotides , Polymerase chain reaction , RNA , Water treatment plants , Effluents , Bacterial RNA , RNA 16S , Bacterium , Detection method , Effluent , Polymer , Probe , Sampling , Waste treatment , Water treatment , Arcobacter , Arcobacter butzleri , Article , Bacterial cell , Bacterial count , Bacterium culture , Bacterium detection , Campylobacter coli , Campylobacter jejuni , Cell isolation , Cell viability , Citrobacter freundii , Comparative study , Controlled study , Direct viable count , Enterobacter cloacae , Enterococcus faecalis , Escherichia coli , Fluorescence in situ hybridization , Helicobacter pylori , Listeria innocua , Listeria ivanovii , Micrococcus luteus , Nonhuman , Oligonucleotide probe , Priority journal , Pseudomonas aeruginosa , RNA sequence , Salmonella enterica , Spain
Copyrigths: Reserva de todos los derechos
Source:
Water Research. (issn: 0043-1354 )
DOI: 10.1016/j.watres.2011.06.015
Publisher:
IWA Publishing
Publisher version: http://dx.doi.org/10.1016/j.watres.2011.06.015
Project ID:
info:eu-repo/grantAgreement/MICINN//AGL2008-05275-C03-02/ES/ANALISIS INTEGRADO DE RIESGOS EN AGUA Y ALIMENTOS PARA BACTERIAS PATOGENAS EMERGENTES POR METODOS MOLECULARES: DESINFECCION, SUPERVIVENCIA Y TRAZABILIDAD/
Thanks:
This work was supported by the grant AGL2008-05275-C03-02 (national and FEDER funds) from Ministerio de Ciencia e Innovacion, Spain.
Type: Artículo

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