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Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR

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Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR

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dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Ballesteros, Lorena es_ES
dc.contributor.author García Hernández, Jorge es_ES
dc.contributor.author Santiago Cuéllar, Paula es_ES
dc.contributor.author González Pellicer, Ana es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.date.accessioned 2016-05-02T11:13:43Z
dc.date.available 2016-05-02T11:13:43Z
dc.date.issued 2011-10
dc.identifier.issn 0043-1354
dc.identifier.uri http://hdl.handle.net/10251/63333
dc.description.abstract Listeria monocytogenes detection in wastewater can be difficult because of the large amount of background microbiota and the presence of viable but non-culturable forms in this environment. The aim of this study was to evaluate a Fluorescent In Situ Hybridization (FISH) assay combined with Direct Viable Count (DVC) method for detecting viable L. monocytogenes in wastewater samples, as an alternative to conventional culture methods. 16S rRNA sequence data were used to design a specific oligonucleotide probe. In order to assess the suitability of the method, the assays were performed on naturally (n = 87) and artificially (n = 14) contaminated samples and results were compared to those obtained with the isolation of cells on selective media and with a PCR method. The detection limit of FISH and PCR assays was 10 4 cells/mL without enrichment and 10 cells/mL after enrichment. A total of 47 samples, including 3 samples from effluent sites, yielded FISH positive results for L. monocytogenes. Using DVC-FISH technique, the presence of viable L. monocytogenes cells was detected in 23 out of these 47 FISH positive wastewater samples. PCR and culture methods yielded 27 and 23 positive results, respectively. According to these results, FISH technique has the potential to be used as a sensitive method for the detection and enumeration of L. monocytogenes in environmental wastewater samples. © 2011 Elsevier Ltd. es_ES
dc.description.sponsorship This work was supported by the grant AGL2008-05275-C03-02 (national and FEDER funds) from Ministerio de Ciencia e Innovacion, Spain. en_EN
dc.language Inglés es_ES
dc.publisher IWA Publishing es_ES
dc.relation Ministerio de Ciencia e Innovacion, Spain AGL2008-05275-C03-02 es_ES
dc.relation.ispartof Water Research es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Listeria monocytogenes es_ES
dc.subject PCR es_ES
dc.subject Wastewater es_ES
dc.subject Wastewater samples es_ES
dc.subject Wastewater treatment plants es_ES
dc.subject Assays es_ES
dc.subject Cell culture es_ES
dc.subject Diseases es_ES
dc.subject Fluorescence es_ES
dc.subject Listeria es_ES
dc.subject Oligonucleotides es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject RNA es_ES
dc.subject Water treatment plants es_ES
dc.subject Effluents es_ES
dc.subject Bacterial RNA es_ES
dc.subject RNA 16S es_ES
dc.subject Bacterium es_ES
dc.subject Detection method es_ES
dc.subject Effluent es_ES
dc.subject Polymer es_ES
dc.subject Probe es_ES
dc.subject Sampling es_ES
dc.subject Waste treatment es_ES
dc.subject Water treatment es_ES
dc.subject Arcobacter es_ES
dc.subject Arcobacter butzleri es_ES
dc.subject Article es_ES
dc.subject Bacterial cell es_ES
dc.subject Bacterial count es_ES
dc.subject Bacterium culture es_ES
dc.subject Bacterium detection es_ES
dc.subject Campylobacter coli es_ES
dc.subject Campylobacter jejuni es_ES
dc.subject Cell isolation es_ES
dc.subject Cell viability es_ES
dc.subject Citrobacter freundii es_ES
dc.subject Comparative study es_ES
dc.subject Controlled study es_ES
dc.subject Direct viable count es_ES
dc.subject Enterobacter cloacae es_ES
dc.subject Enterococcus faecalis es_ES
dc.subject Escherichia coli es_ES
dc.subject Fluorescence in situ hybridization es_ES
dc.subject Helicobacter pylori es_ES
dc.subject Listeria innocua es_ES
dc.subject Listeria ivanovii es_ES
dc.subject Micrococcus luteus es_ES
dc.subject Nonhuman es_ES
dc.subject Oligonucleotide probe es_ES
dc.subject Priority journal es_ES
dc.subject Pseudomonas aeruginosa es_ES
dc.subject RNA sequence es_ES
dc.subject Salmonella enterica es_ES
dc.subject Spain es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.watres.2011.06.015
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Moreno Trigos, MY.; Ballesteros, L.; García Hernández, J.; Santiago Cuellar, P.; González Pellicer, A.; Ferrús Pérez, MA. (2011). Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR. Water Research. 45(15):4634-4640. doi:10.1016/j.watres.2011.06.015 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.watres.2011.06.015 es_ES
dc.description.upvformatpinicio 4634 es_ES
dc.description.upvformatpfin 4640 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 45 es_ES
dc.description.issue 15 es_ES
dc.relation.senia 210882 es_ES


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