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Browsing by UPV Unit "Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia"

Now showing items 1-20 of 1744

"Desarrollo de un protocolo para el análisis metabolómico de tejidos tumorales vesicales mediante espectrometría de masas"

Sánchez Sánchez, Rafael (Universitat Politècnica de València, 2016-09-06)

[EN] This project aims to develop a protocol for the metabolic analysis of bladder tumoral tissues by mass spectrometry. For this purpose, we will study with 9 samples of tissue from the same patient, what kind of solvent ...
25 años en la mejora genética para resistencia a plagas y enfermedades

Pérez de Castro, Ana María; Díez Niclós, Mª José Teresa de Jesús (Phytoma España, 2013-04)

En los últimos 25 años se han producido espectaculares avances en el campo de la mejora genética para la resistencia a plagas y enfermedades, tanto en lo referente al conocimiento y manejo de los patógenos, como al desarrollo ...
A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L

Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Corredor, E.; Raska, I.; Testillano, P. S.; Risueno, M.C. (Elsevier, 2011-05-15)

After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. ...
A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein

Julve Parreño, Jose Manuel; Gandia Fernàndez, Antoni; Fernández Del Carmen, María Asunción; Sarrion-Perdigones, Alejandro; Castelijns, Bas; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer Verlag (Germany), 2013-04)

[EN] New evidence is emerging which indicates that population variants in plant virus infections are not uniformly distributed along the plant, but structured in a mosaic-like pattern due to limitation to the superinfection ...
A collection of enhancer trap insertional mutants for functional genomics in tomato

Pérez-Martín, Fernando; Yuste-Lisbona, Fernando J.; Pineda Chaza, Benito José; Angarita-Díaz, María Pilar; García Sogo, Begoña; Antón Martínez, María Teresa; SANCHEZ MARTÍN-SAUCEDA, SIBILLA; Gimenez, Estela; Atarés Huerta, Alejandro; Fernández-Lozano, Antonia; Ortiz-Atienza, Ana; García-Alcázar, Manuel; Castañeda, Laura; Fonseca, Rocio; Capel, Carmen; Goergen, Geraldine; Sanchez-Lopez, Jorge; Quispe, Jorge L.; Capel, Juan; Angosto, Trinidad; Moreno Ferrero, Vicente; Lozano, Rafael (Blackwell Publishing, 2017)

[EN] With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of ...
A combination of direct viable count and fluorescence in situ hibridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

García Hernández, Jorge; Moreno Trigos, Mª Yolanda; Amorocho Cruz, Claudia Milena; Hernández Pérez, Manuel (Wiley, 2011-03)

[EN] Aims: We have developed a direct viable count (DVC)-FISH procedure for quickly and easily discriminating between viable and nonviable cells of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus ...
A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays

Gonzalez-Ibeas, Daniel; Blanca Postigo, José Miguel; Cañizares Sales, Joaquín; Truniger, Veronica; Aranda, Miguel A. (Springer Verlag (Germany), 2012-10)

[EN] DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays ...
A cytochrome P450 regulates a domestication trait in cultivated tomato

Chakrabarti, Manohar; Zhang, Na; Sauvage, Christopher; Muños, Stéphane; Blanca Postigo, José Miguel; Cañizares Sales, Joaquín; Díez Niclós, Mª José Teresa de Jesús; Schneider, Rhiannon; Mazourek, Michael; McClead, Jammi; Causse, Mathilde; Van Der Knaap, Esther Klazina Maria (National Academy of Sciences, 2013-10-15)

Domestication of crop plants had effects on human lifestyle and agriculture. However, little is known about the underlying molecular mechanisms accompanying the changes in fruit appearance as a consequence of selection by ...
A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components

Peirats-Llobet, Marta; Han, Soon-Ki; González Guzmán, Miguel; Jeong, Cheol Woong; Rodríguez Solovey, Leisa Natacha; Belda Palazón, Borja; Wagner, Doris; Rodríguez Egea, Pedro Luís (Oxford University Press (OUP), 2016-01-04)

[EN] Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated ...
A dominant-negative form of Arabidopsis AP-3 #-adaptin improves intracellular pH homeostasis

Niñoles Rodenes, Regina; Rubio, Lourdes; García Sánchez, María J.; Fernandez, Jose A.; Bueso Ródenas, Eduardo; Alejandro Martínez, Santiago; Serrano Salom, Ramón (Wiley, 2013-05)

[EN] Intracellular pH (pHi) is a crucial parameter in cellular physiology but its mechanisms of homeostasis are only partially understood. To uncover novel roles and participants of the pHi regulatory system, we have ...
A Drosophila model of GDAP1 function reveals the involvement of insulin signalling in the mitochondria-dependent neuromuscular degeneration

Lopez del Amo, V; Palomino-Schätzlein, Martina; Seco-Cervera, Marta; Garcia-Gimenez, José Luis; Pallardó-Calatayud, Federico V.; Pineda-Lucena, Antonio; Galindo-Orozco, Máximo Ibo (Elsevier, 2017)

[EN] Charcot-Marie-Tooth disease is a rare peripheral neuropathy for which there is no specific treatment. Some forms of Charcot-Marie-Tooth are due to mutations in the GDAP1 gene. A striking feature of mutations in GDAP1 ...
A dynamic snapshot of bud dormancy in peach

Lloret Compañ, Alba (Universitat Politècnica de València, 2020-11-02)

[EN] The general aim of this thesis is to study the dormancy process from a molecular point of view identifying mechanisms and targeting genes that control it. In order to do that we have focused on the study of three genes ...
A forward genetic approach in Arabidopsis thaliana identifies a RING-type ubiquitin ligase as a novel determinant of seed longevity

Bueso Ródenas, Eduardo; Ibanez, Carla; Sayas, Enric; Muñoz Bertomeu, Jesús; González Guzmán, Miguel; Rodríguez Egea, Pedro Luís; Serrano Salom, Ramón (Elsevier, 2014-02)

[EN] Seed longevity is important to preserve crops and wild plants and it is limited by progressive cellular damage (aging) during storage. The induction of cellular stress defenses and the formation of the seed coat are ...
A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance

Martínez Macías, Félix; Arif, Anjuman; González Nebauer, Sergio; Bueso Ródenas, Eduardo; Ali, Rashid; Montesinos De Lago, Consuelo; Brunaud, Veronique; Muñoz Bertomeu, Jesús; Serrano Salom, Ramón (Springer Verlag (Germany), 2015)

[EN] A fungal gene encoding a transcription factor is expressed from its own promoter in Arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. Horizontal gene ...
A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae

Barreto, Lina; Canadell, David; Petrezselyova, Silvia; Navarrete, Clara; Maresova, Lydie; Pérez Valle, Jorge; Herrera, Rito; Olier, Ivan; Giraldo, Jesus; Sychrova, Hana; Yenush, Lynne; Ramos, Jose; Arino, Joaquin (American Society for Microbiology, 2011)

[EN] Potassium homeostasis is crucial for living cells. In the yeast Saccharomyces cerevisiae, the uptake of potassium is driven by the electrochemical gradient generated by the Pma1 H +-ATPase, and this process represents ...
A mechanism of growth inhibition by abscisic acid in germinating seeds of Arabidopsis thaliana based on inhibition of plasma membrane H+-ATPase and decreased cytosolic pH, K+, and anions

Planes Ferrer, Mª Dolores; Niñoles Rodenes, Regina; Rubio, Lourdes; Bissoli, Gaetano; Bueso Ródenas, Eduardo; Garcia-Sanchez, Maria J.; Alejandro Martínez, Santiago; González Guzmán, Miguel; Hedrich, Rainer; Rodríguez Egea, Pedro Luís; Fernandez, Jose A; Serrano Salom, Ramón (Oxford University Press (OUP), 2015-02)

[EN] The stress hormone abscisic acid (ABA) induces expression of defence genes in many organs, modulates ion homeostasis and metabolism in guard cells, and inhibits germination and seedling growth. Concerning the ...
A mechanistic study on the potential of quinolinium salts as photocatalysts for the abatement of chlorinated pollutants

Martínez-Haya, Rebeca; Sabater Marco, Consuelo; Castillo López, María-Ángeles; Miranda Alonso, Miguel Ángel; Marín García, Mª Luisa (Elsevier, 2018-06)

[EN] Photocatalytic degradation of three highly chlorinated contaminants, namely 2,4,6-trichlorophenol (TCP), 2,4,6-trichloroanisole (TCA) and 5-chloro-2-(2,4-dichlorophenoxy)phenol (triclosan, TCS) has been investigated ...
A Method for a Fast Evaluation of the Biostimulant Potential of Different Natural Extracts for Promoting Growth or Tolerance against Abiotic Stress

SAPORTA BON, RUBÉN; Bou, C.; Frías, V.; Mulet, José Miguel (MDPI, 2019-03-20)

[EN] Under adverse environmental conditions, biostimulants can help crops withstand abiotic stress while increasing productivity. We have designed a sequential system based on two different biological model organisms¿the ...
A Methodological Approach for Testing the Viability of Seeds Stored in Short-Term Seed Banks

Forte Gil, J.A.; Yabor, L.; Bellido Nadal, A.; Collado Cerveró, Francisco; Ferrer-Gallego, Pablo; Vicente, Oscar; Boscaiu, Monica (AcademicPres (EAP) Publishing House, 2017)

[EN] Efficient management of `active¿ seed banks ¿ specifically aimed at the short-term storage at room temperature of seeds to be used locally in conservation/regeneration programmes of endemic or endangered plant species ...
A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Vázquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernández Del Carmen, María Asunción; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer (Biomed Central Ltd.), 2016)

[EN] Background: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the ...