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Browsing by UPV Unit "Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia"

Now showing items 1-20 of 1318

25 años en la mejora genética para resistencia a plagas y enfermedades

Pérez de Castro, Ana María; Díez Niclós, Mª José Teresa de Jesús (Phytoma España, 2013-04)

En los últimos 25 años se han producido espectaculares avances en el campo de la mejora genética para la resistencia a plagas y enfermedades, tanto en lo referente al conocimiento y manejo de los patógenos, como al desarrollo ...
A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L.

Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Corredor, E.; Raska, I.; Testillano, P. S.; Risueno, M.C. (Elsevier, 2011-05-15)

After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. ...
A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein.

Julve Parreño, Jose Manuel; Gandia Fernàndez, Antoni; Fernández Del Carmen, María Asunción; Sarrion-Perdigones, Alejandro; Castelijns, Bas; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer Verlag (Germany), 2013-04)

[EN] New evidence is emerging which indicates that population variants in plant virus infections are not uniformly distributed along the plant, but structured in a mosaic-like pattern due to limitation to the superinfection ...
A combination of direct viable count and fluorescence in situ hibridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

García Hernández, Jorge; Moreno Trigos, Mª Yolanda; Amorocho Cruz, Claudia Milena; Hernández Pérez, Manuel (Wiley, 2011-03)

[EN] Aims: We have developed a direct viable count (DVC)-FISH procedure for quickly and easily discriminating between viable and nonviable cells of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus ...
A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays

Gonzalez-Ibeas, Daniel; Blanca Postigo, José Miguel; Cañizares Sales, Joaquín; Truniger, Veronica; Aranda, Miguel A. (Springer Verlag (Germany), 2012-10)

[EN] DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays ...
A cytochrome P450 regulates a domestication trait in cultivated tomato

Chakrabarti, Manohar; Zhang, Na; Sauvage, Christopher; Muños, Stéphane; Blanca Postigo, José Miguel; Cañizares Sales, Joaquín; Díez Niclós, Mª José Teresa de Jesús; Schneider, Rhiannon; Mazourek, Michael; McClead, Jammi; Causse, Mathilde; Van Der Knaap, Esther Klazina Maria (National Academy of Sciences, 2013-10-15)

Domestication of crop plants had effects on human lifestyle and agriculture. However, little is known about the underlying molecular mechanisms accompanying the changes in fruit appearance as a consequence of selection by ...
A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components

Peirats-Llobet, Marta; Han, Soon-Ki; González Guzmán, Miguel; Jeong, Cheol Woong; Rodríguez Solovey, Leisa Natacha; Belda Palazón, Borja; Wagner, Doris; Rodríguez Egea, Pedro Luís (Oxford University Press (OUP), 2016-01-04)

[EN] Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated ...
A dominant-negative form of Arabidopsis AP-3 #-adaptin improves intracellular pH homeostasis.

Niñoles Rodenes, Regina; Rubio, Lourdes; García Sánchez, María J.; Fernandez, Jose A.; Bueso Ródenas, Eduardo; Alejandro Martínez, Santiago; Serrano Salom, Ramón (Wiley, 2013-05)

[EN] Intracellular pH (pHi) is a crucial parameter in cellular physiology but its mechanisms of homeostasis are only partially understood. To uncover novel roles and participants of the pHi regulatory system, we have ...
A forward genetic approach in Arabidopsis thaliana identifies a RING-type ubiquitin ligase as a novel determinant of seed longevity

Bueso Ródenas, Eduardo; Ibanez, Carla; Sayas, Enric; Muñoz Bertomeu, Jesús; González Guzmán, Miguel; Rodríguez Egea, Pedro Luís; Serrano Salom, Ramón (Elsevier, 2014-02)

[EN] Seed longevity is important to preserve crops and wild plants and it is limited by progressive cellular damage (aging) during storage. The induction of cellular stress defenses and the formation of the seed coat are ...
A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance

Martínez Macías, Félix; Arif, Anjuman; González Nebauer, Sergio; Bueso Ródenas, Eduardo; Ali, Rashid; Montesinos De Lago, Consuelo; Brunaud, Veronique; Muñoz Bertomeu, Jesús; Serrano Salom, Ramón (Springer Verlag (Germany), 2015)

[EN] A fungal gene encoding a transcription factor is expressed from its own promoter in Arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. Horizontal gene ...
A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae

Barreto, Lina; Canadell, David; Petrezselyova, Silvia; Navarrete, Clara; Maresova, Lydie; Pérez Valle, Jorge; Herrera, Rito; Olier, Ivan; Giraldo, Jesus; Sychrova, Hana; Yenush, Lynne; Ramos, Jose; Arino, Joaquin (American Society for Microbiology, 2011)

[EN] Potassium homeostasis is crucial for living cells. In the yeast Saccharomyces cerevisiae, the uptake of potassium is driven by the electrochemical gradient generated by the Pma1 H +-ATPase, and this process represents ...
A mechanism of growth inhibition by abscisic acid in germinating seeds of Arabidopsis thaliana based on inhibition of plasma membrane H+-ATPase and decreased cytosolic pH, K+, and anions.

Planes Ferrer, Mª Dolores; Niñoles Rodenes, Regina; Rubio, Lourdes; Bissoli, Gaetano; Bueso Ródenas, Eduardo; Garcia-Sanchez, Maria J.; Alejandro Martínez, Santiago; González Guzmán, Miguel; Hedrich, Rainer; Rodríguez Egea, Pedro Luís; Fernandez, Jose A; Serrano Salom, Ramón (Oxford University Press (OUP), 2015-02)

[EN] The stress hormone abscisic acid (ABA) induces expression of defence genes in many organs, modulates ion homeostasis and metabolism in guard cells, and inhibits germination and seedling growth. Concerning the ...
A Methodological Approach for Testing the Viability of Seeds Stored in Short-Term Seed Banks

Forte Gil, J.A.; Yabor, L.; Bellido Nadal, A.; Collado Cerveró, Francisco; Ferrer-Gallego, Pablo; Vicente, Oscar; Boscaiu, Monica (AcademicPres (EAP) Publishing House, 2017)

[EN] Efficient management of `active¿ seed banks ¿ specifically aimed at the short-term storage at room temperature of seeds to be used locally in conservation/regeneration programmes of endemic or endangered plant species ...
A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Vázquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernández Del Carmen, María Asunción; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer (Biomed Central Ltd.), 2016)

[EN] Background: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the ...
A multilevel, developmentally controlled gene engineering strategy for tomato fortification and protection

Cocaliadis Caisson, María Florencia (2017-11-03)

Plastids are the cellular organelles where many of the visual, health and flavor-related metabolites are produced and stored in the fruit, and therefore are valuable components for consumers and breeders. The more sugar ...
A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits

Perpiñá Martín, Gorka; Esteras Gómez, Cristina; Gibon, Y.; Monforte Gilabert, Antonio José; Picó Sirvent, María Belén (Springer (Biomed Central Ltd.), 2016)

[EN] Background: Genomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, ...
A new methodology to assess the performance of AOPs in complex samples: Application to the degradation of phenolic compounds by O3 and O3/UV-A Vis

García-Ballesteros, Sara; Mora Carbonell, Margarita; Vicente Candela, Rafael; Vercher Pérez, Rosa Francisca; Sabater Marco, Consuelo; Castillo López, María-Ángeles; Amat Payá, Ana María; Arqués Sanz, Antonio (Elsevier, 2019)

[EN] A methodology combining experimental design methodology, liquid chromatography, excitation emission matrixes (EEM) and bioassays has been applied to study the performance of O3 and O3/UVA-vis in the treatment of a ...
A new role for green leaf volatile esters in tomato stomatal defense against Pseudomonas syringe pv. tomato

López-Gresa, María Pilar; Payá, C.; Ozáez-Martínez, Miguel; Rodrigo Bravo, Ismael; Conejero Tomás, Vicente; Klee, H; Belles Albert, José Mª; Lisón, Purificación (Frontiers Media SA, 2018)

[EN] The volatile esters of (Z)-3-hexenol with acetic, propionic, isobutyric, or butyric acids are synthesized by alcohol acyltransferases (AAT) in plants. These compounds are differentially emitted when tomato plants are ...
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae

López-Gresa, María Pilar; Lisón, Purificación; Campos Beneyto, Laura; Rodrigo Bravo, Ismael; Rambla Nebot, Jose Luis; Granell Richart, Antonio; Conejero Tomás, Vicente; Belles Albert, José Mª (Frontiers Media SA, 2017)

[EN] Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These compounds may perform direct defensive functions, i. e., acting as ...
A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato

Lisón Párraga, María Purificación; Tarraga Herrero, Susana; López Gresa, Mª Pilar; Sauri Ferrando, Asunción; Torres Vidal, Cristina; Campos Beneyto, Laura; Belles Albert, José Mª; Conejero Tomás, Vicente; Rodrigo Bravo, Ismael (Wiley-Blackwell, 2013-03)

Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected ...