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Browsing by Subject "Microarray"

Now showing items 1-20 of 20

A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays

Gonzalez-Ibeas, Daniel; Blanca Postigo, José Miguel; Cañizares Sales, Joaquín; Truniger, Veronica; Aranda, Miguel A. (Springer Verlag (Germany), 2012-10)

[EN] DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays ...
A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by Prunus necrotic ringspot virus and Peach latent mosaic viroid

Herranz Gordo, Maria Del Carmen; Niehl, A; Rosales, Marlene; Fiore, Nicola; Zamorano, A; Granell Richart, Antonio; Pallás Benet, Vicente (BioMed Central, 2013-05-28)

[EN] Background: Microarray profiling is a powerful technique to investigate expression changes of large amounts of genes in response to specific environmental conditions. The majority of the studies investigating gene ...
Bayesian recursive mixed linear model for gene expression analyses with continuous covariates

Casellas, J.; Ibáñez-Escriche, Noelia (American Society of Animal Science, 2012-01)

[EN] The analysis of microarray gene expression data has experienced a remarkable growth in scientific research over the last few years and is helping to decipher the genetic background of several productive traits. ...
Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties

Saladie, Montserrat; Cañizares Sales, Joaquín; Michael A. Phillips; Rodriguez Concepcion, Manuel; Larrigaudière, Christian; Gibon, Yves; Stitt, Mark; Lunn, John Edward; Garcia Mas, Jordi (BioMed Central, 2015-06-09)

[EN] Background: In climacteric fruit-bearing species, the onset of fruit ripening is marked by a transient rise in respiration rate and autocatalytic ethylene production, followed by rapid deterioration in fruit quality. ...
Desarrollo de inmunoensayos para antibióticos en microplaca y en disco compacto aplicados a la determinación multirresiduo de contaminantes

Gallego Iglesias, Ester (2012-02-17)

Los antibióticos son ampliamente utilizados en veterinaria, produciendo beneficios inmensos, pero también conllevan riesgos medioambientales debidos a la aparición de residuos "incontrolados" que pueden desencadenar ...
Desarrollo de metodologías de anclaje de anticuerpos basadas en reacciones de click-chemistry. Aplicación a la detección de marcadores cardíacos

Poveda Úbeda, Adrián (2016-09-02)

[ES] La detección temprana de enfermedades muchas veces se basa en la medición de una o varias proteínas y/o en el análisis genético. El formato microarray es un buen candidato para aplicarlo a los dispositivos de detección ...
Desarrollo de microarrays de altas prestaciones basados en atrapamiento con un hidrogel. Aplicación a inmunoensayo y ensayos genéticos.

Guerrero Picó, Ismael (2015-09-04)

[ES] La detección temprana de enfermedades muchas veces se basa en la medición de una o varias proteínas y/o en el análisis genético. El formato microarray es un buen candidato para aplicarlo a los dispositivos de detección ...
Desarrollo de microinmunoensayos para la determinación de endotoxinas

Moriana Martínez, Santiago (2015-10-06)

[ES] En la industria agroalimentaria el análisis microbiológico es una actividad necesaria para poder comercializar productos sin riesgo para la salud del consumidor. Algunos microorganismos son capaces de provocar ...
Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Tamarit López, Jesús; Morais Ezquerro, Sergi Beñat; Puchades Pla, Rosa; Maquieira Catala, Ángel (Elsevier, 2011-01-15)

Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are ...
Ensayo in vitro para la detección multiplexada de alergias alimentarias.

Casañ Raga, Natalia (2018-09-17)

[ES] Los alérgenos alimentarios pueden provocar reacciones adversas de hipersensibilidad de tipo I. Este proceso presenta una elevada prevalencia que puede afectar a la población sensibilizada vía contacto, ingestión o ...
Ensayos de hibridación de ADN en formato microarray para la detección de E. coli: Estudio de funcionalizaciones superficiales que mejoren las prestaciones de sensibilidad y especificidad

Cerdá Doñate, Elisa (2014-09-19)

[ EN ] The present work arises from the current need to develop new diagnosis strategies in the medical sector which allow for a fast and precise detection of diverse infectious pathologies. Furthermore, it looks to ...
Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products

Tortajada-Genaro, Luis Antonio; Rodrigo, Alejandro; Hevia, Elizabeth; Mena, Salvador; Niñoles Rodenes, Regina; Maquieira Catala, Ángel (Springer Verlag (Germany), 2015-09)

[EN] Highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food ...
One-pot isothermal DNA amplification Hybridisation and detection by a disc-based method

Santiago Felipe, Sara; Tortajada-Genaro, Luis Antonio; Morais, Sergi; Puchades, Rosa; Maquieira Catala, Ángel (Elsevier, 2014-12-01)

[EN] An integrated sensor comprising isothermal DNA amplification and in situ detection is presented. The method principle is based on recombinase polymerase amplification (RPA) and detection in the microarray format by ...
Ploidy and Gene expression in clementine

Niñoles Rodenes, Regina; Aleza, Pablo; Castillo, Mª Cruz; Navarro Lucas, Luis; Ancillo, Gema (International Society for Horticultural Science (ISHS), 2015-01)

Polyploidy has long been recognized as an important force in the evolution of flowering plants and often results in novel phenotypes having advantages in adaptation and major interest for agriculture. Many crops, including ...
Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms

Pons Puig, Clara; Dagar, Anurag; Martí Ibáñez, María Cristina; Singh, Vikram; Crisosto, Carlos H.; Friedman, Haya; Lurie, Susan; Granell Richart, Antonio (BioMed Central, 2015-03-26)

Background: Cold storage induces chilling injury (CI) disorders in peach fruit (woolliness/mealiness, flesh browning and reddening/bleeding) manifested when ripened at shelf life. To gain insight into the mechanisms ...
Primer design for SNP genotyping based on allele-specific amplification Application to organ transplantation pharmacogenomics

Tortajada-Genaro, Luis Antonio; Puchades Pla, Rosa; Maquieira Catala, Ángel (Elsevier, 2017-03-20)

[EN] Diagnostic methods based on single nucleotide polymorphism (SNP) biomarkers are essential for the real adoption of personalized medicine. Allele specific amplification in a homogeneous format or combined to microarray ...
Reducción selectiva de anticuerpos para su anclaje a soportes sólidos mediante la reacción de acoplamiento tiol-eno. Aplicación a la detección de Mioglobina con y sin marcaje.

Sayas Cortés, Marina (2018-09-18)

[ES] Las enfermedades cardiovasculares (ECV) constituyen la principal causa de muerte en la unión europea, representando un 40% de los fallecimientos totales. Es por ello que disponer de dispositivos para su diagnóstico ...
Respuesta de los frutos cítricos a las bajas temperaturas: estudio mediante micromatrices

Royo Brun, Carolina (2010-10-13)

En esta Tesis Doctoral se ha abordado el estudio de la respuesta al frío en el flavedo de frutos cítricos, simulando tratamientos cuarentenarios requeridos para evitar la propagación de plagas con la exportación. ...
Thiol-click photochemistry for surface functionalization applied to optical biosensing

Bañuls Polo, María-José; González Martínez, Miguel Ángel; Sabek, Jad; García-Rupérez, Jaime; Maquieira Catala, Ángel (Elsevier, 2019-07-04)

[EN] In the field of biosensing, suitable procedures for efficient probes immobilization are of outmost importance. Here we present different light-based strategies to promote the covalent attachment of thiolated capture ...
Variability-specific differential gene expression across reproductive stages in sows

Casellas, J.; Martinez Giner, M.; Pena, R.; Balcells, I.; Fernández-Rodríguez, A .; Ibañez Escriche, Noelia; Noguera, J.L. (Cambridge University Press, 2013)

[EN] Differential gene expression analyses typically focus on departures across mathematical expectations (i.e. mean) from two or more groups of microarrays, without considering alternative patterns of departure. Nevertheless, ...